CPLM-000657

Genbank Nucleotide ID

Cytochrome b5 type B

Protein Synonyms/Alias

Cytochrome b5 outer mitochondrial membrane isoform

Homo sapiens (Human)

Position	Peptide	Type	References
30	YRLEEVAKRNSLKEL	acetylation	[1]
30	YRLEEVAKRNSLKEL	ubiquitination	[2, 3, 4, 5, 6]
35	VAKRNSLKELWLVIH	ubiquitination	[2, 3, 4, 5, 6, 7, 8]
88	SDAREMLKQYYIGDI	ubiquitination	[2, 3, 5, 6]
101	DIHPSDLKPESGSKD	ubiquitination	[3, 5]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases (By similarity).

DOMAIN 20 96 Cytochrome b5 heme-binding.
METAL 55 55 Iron (heme axial ligand) (By similarity).
METAL 79 79 Iron (heme axial ligand) (By similarity).
MOD_RES 30 30 N6-acetyllysine.
MOD_RES 33 33 Phosphoserine (By similarity).

3D-structure; Acetylation; Complete proteome; Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
GO:0020037; F:heme binding; IDA:UniProtKB.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.

IPR001199; Cyt_B5-like_heme/steroid-bd.
IPR018506; Cyt_B5_heme-BS.

PS00191; CYTOCHROME_B5_1
PS50255; CYTOCHROME_B5_2

PR00363; CYTOCHROMEB5.