UniProt Accession

 UBC8_YEAST; P28263; D3DLN7 

Genbank Protein ID

 M65083; U18530; Z29329; BK006939 

Genbank Nucleotide ID

 AAB64489.1; CAA82526.1; DAA07641.1 

Protein Name

 Ubiquitin-conjugating enzyme E2-24 kDa 

Protein Synonyms/Alias

 Glucose-induced degradation protein 3; Ubiquitin carrier protein; Ubiquitin-protein ligase 

Gene Name

 UBC8 

Gene Synonyms/Alias

 GID3; YEL012W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
62	LPDNYPYKSPSIGFV	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Has a role in the negative regulation of gluconeogenesis. Required for proteasome-dependent catabolite degradation of fructose-1,6- bisphosphatase (FBPase). 

Sequence Annotation

 ACT_SITE 85 85 Glycyl thioester intermediate (By  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 218 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0045721; P:negative regulation of gluconeogenesis; IMP:SGD.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD.
 GO:0006513; P:protein monoubiquitination; TAS:SGD.
 GO:0000209; P:protein polyubiquitination; TAS:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IMP:SGD. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS