CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020282
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Uncharacterized protein C1orf43 
Protein Synonyms/Alias
 Hepatitis C virus NS5A-transactivated protein 4; HCV NS5A-transactivated protein 4; Protein NICE-3; S863-3 
Gene Name
 C1orf43 
Gene Synonyms/Alias
 NICE3; NS5ATP4; HSPC012 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59HNAPKDLKEEIDIRLubiquitination[1]
73LSRVQDIKYEPQLLAubiquitination[1, 2, 3, 4]
105YNYLYRMKALDAIRTubiquitination[1]
130HPRSLMGKNFRSYLLubiquitination[1]
151TPFKGVRKALIDTLLubiquitination[1, 2, 5]
191SELATAVKARIGSSQubiquitination[1, 2, 3, 4, 5, 6]
229TYLPSSQKSKRAKHFubiquitination[4]
234SQKSKRAKHFLELKSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 253 AA 
Protein Sequence
MASGSNWLSG VNVVLVMAYG SLVFVLLFIF VKRQIMRFAM KSRRGPHVPV GHNAPKDLKE 60
EIDIRLSRVQ DIKYEPQLLA DDDARLLQLE TQGNQSCYNY LYRMKALDAI RTSEIPFHSE 120
GRHPRSLMGK NFRSYLLDLR NTSTPFKGVR KALIDTLLDG YETARYGTGV FGQNEYLRYQ 180
EALSELATAV KARIGSSQRH HQSAAKDLTQ SPEVSPTTIQ VTYLPSSQKS KRAKHFLELK 240
SFKDNYNTLE STL 253 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro. 
Interpro
 IPR013328; DH_multihelical.
 IPR010876; NICE-3_prd. 
Pfam
 PF07406; NICE-3 
SMART
  
PROSITE
  
PRINTS