CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023178
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proline-rich protein 12 
Protein Synonyms/Alias
  
Gene Name
 PRR12 
Gene Synonyms/Alias
 KIAA1205 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
101SPSPQGTKAPRFVPLubiquitination[1]
247SPIFCSTKPKKLLKTacetylation[2]
402GTRLEPLKPLKIKLSacetylation[2, 3, 4, 5]
729TRPLHLAKKQETAAVubiquitination[6]
730RPLHLAKKQETAAVCubiquitination[6]
1063LYLPPMRKIDGLLNEubiquitination[7]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 DNA_BIND 347 359 A.T hook 1.
 DNA_BIND 381 393 A.T hook 2.
 MOD_RES 44 44 Phosphoserine.
 MOD_RES 256 256 Phosphoserine.
 MOD_RES 402 402 N6-acetyllysine.
 MOD_RES 483 483 Phosphothreonine.
 MOD_RES 487 487 Phosphoserine.
 MOD_RES 560 560 Phosphoserine.
 MOD_RES 561 561 Phosphoserine.
 MOD_RES 566 566 Phosphoserine.
 MOD_RES 740 740 Phosphothreonine.
 MOD_RES 747 747 Phosphoserine.
 MOD_RES 1104 1104 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1215 AA 
Protein Sequence
MEQLPGVPPG PPHPVRPPPP PPPPMPLQLE AHLRSHGLEP AAPSPRLRPE ESLDPPGAMQ 60
ELLGALEPLP PAPGDTGVGP PNSEGKDPAG AYRSPSPQGT KAPRFVPLTS ICFPDSLLQD 120
EERSFFPTME EMFGGGAADD YGKAGPPEDE GDPKAGAGPP PGPPAYDPYG PYCPGRASGA 180
GPETPGLGLD PNKPPELPST VNAEPLGLIQ SGPHQAAPPP PPPPPPPPAP ASEPKGGLTS 240
PIFCSTKPKK LLKTSSFHLL RRRDPPFQTP KKLYAQEYEF EADEDKADVP ADIRLNPRRL 300
PDLVSSCRSR PALSPLGDID FCPPNPGPDG PRRRGRKPTK AKRDGPPRPR GRPRIRPLEV 360
PTTAGPASAS TPTDGAKKPR GRGRGRGRKA EEAGGTRLEP LKPLKIKLSV PKAGEGLGTS 420
SGDAISGTDH NSLDSSLTRE KIEAKIKEVE EKQPEMKSGF MASFLDFLKS GKRHPPLYQA 480
GLTPPLSPPK SVPPSVPARG LQPQPPATPA VPHPPPSGAF GLGGALEAAE SEGLGLGCPS 540
PCKRLDEELK RNLETLPSFS SDEEDSVAKN RDLQESISSA ISALDDPPLA GPKDTSTPDG 600
PPLAPAAAVP GPPPLPGLPS ANSNGTPEPP LLEEKPPPTP PPAPTPQPQP PPPPPPPQPA 660
LPSPPPLVAP TPSSPPPPPL PPPPPPAMPS PPPPPPPAAA PLAAPPEEPA APSPEDPELP 720
DTRPLHLAKK QETAAVCGET DEEAGESGGE GIFRERDEFV IRAEDIPSLK LALQTGREPP 780
PIWRVQKALL QKFTPEIKDG QRQFCATSNY LGYFGDAKNR YQRLYVKFLE NVNKKDYVRV 840
CARKPWHRPP VPVRRSGQAK NPVSAGGSSA PPPKAPAPPP KPETPEKTTS EKPPEQTPET 900
AMPEPPAPEK PSLLRPVEKE KEKEKVTRGE RPLRGERATS GRQTRPERSL ATGQPATSRL 960
PKARPTKVKA EPPPKKRKKW LKEAGGNATA GGGPPGSSSD SESSPGAPSE DERAVPGRLL 1020
KTRAMREMYR SYVEMLVSTA LDPDMIQALE DTHDELYLPP MRKIDGLLNE HKKKVLKRLS 1080
LSPALQDALH TFPQLQVEQS GEGSPEEGAV RLRPAGEPYN RKTLSKLKRS VVRAQEFKVE 1140
LEKSGYYTLY HSLHHYKYHT FLRCRDQTLA IEGGAEDLGQ EEVVQQCMRN QPWLEQLFDS 1200
FSDLLAQAQA HSRCG 1215 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro. 
Interpro
 IPR017956; AT_hook_DNA-bd_motif.
 IPR025451; DUF4211. 
Pfam
 PF13926; DUF4211 
SMART
 SM00384; AT_hook 
PROSITE
  
PRINTS