UniProt Accession

 PRP19_RAT; Q9JMJ4; Q3B7C6 

Genbank Protein ID

 AB020022; BC107669 

Genbank Nucleotide ID

 BAA95215.2; AAI07670.1 

Protein Name

 Pre-mRNA-processing factor 19 

Protein Synonyms/Alias

 Neuronal differentiation-related gene protein; PRP19/PSO4 homolog 

Gene Name

 Prpf19 

Gene Synonyms/Alias

 Prp19 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
206	VKPEELSKYRQVASH	acetylation	[1]
261	EQILATLKGHTKKVT	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing (By similarity). 

Sequence Annotation

 DOMAIN 2 68 U-box.
 REPEAT 219 259 WD 1.
 REPEAT 262 301 WD 2.
 REPEAT 304 345 WD 3.
 REPEAT 348 387 WD 4.
 REPEAT 390 429 WD 5.
 REPEAT 433 472 WD 6.
 REPEAT 473 503 WD 7.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 122 122 N6-acetyllysine (By similarity).
 MOD_RES 261 261 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; DNA repair; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 504 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005811; C:lipid particle; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:Compara.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0000974; C:Prp19 complex; ISS:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0000151; C:ubiquitin ligase complex; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IEA:Compara.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0001833; P:inner cell mass cell proliferation; IEA:Compara.
 GO:0008610; P:lipid biosynthetic process; IEA:Compara.
 GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:0048711; P:positive regulation of astrocyte differentiation; IEA:Compara.
 GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IEA:Compara.
 GO:0000245; P:spliceosomal complex assembly; IEA:Compara. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR013915; Pre-mRNA_splic_Prp19.
 IPR000772; Ricin_B_lectin.
 IPR003613; Ubox_domain.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF08606; Prp19
 PF04564; U-box
 PF00400; WD40 

SMART

 SM00504; Ubox
 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.