UniProt Accession

 PAK4_MOUSE; Q8BTW9; Q6ZPX0; Q80Z97; Q9CS71 

Genbank Protein ID

 AY217016; AK129298; AK017713; AK088512; BC048238 

Genbank Nucleotide ID

 AAO61496.1; BAC98108.2; BAB30889.1; BAC40396.1; AAH48238.1 

Protein Name

 Serine/threonine-protein kinase PAK 4 

Protein Synonyms/Alias

 p21-activated kinase 4; PAK-4 

Gene Name

 Pak4 

Gene Synonyms/Alias

 Kiaa1142 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
51	EESARRPKPLIDPAC	ubiquitination	[1]
548	HKASPSLKGFLDRLL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. 

Sequence Annotation

 DOMAIN 11 24 CRIB.
 DOMAIN 323 574 Protein kinase.
 NP_BIND 329 337 ATP (By similarity).
 REGION 25 322 Linker.
 ACT_SITE 442 442 Proton acceptor (By similarity).
 BINDING 352 352 ATP (By similarity).
 MOD_RES 41 41 Phosphoserine (By similarity).
 MOD_RES 104 104 Phosphoserine (By similarity).
 MOD_RES 148 148 Phosphoserine (By similarity).
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 187 187 Phosphothreonine (By similarity).
 MOD_RES 195 195 Phosphoserine (By similarity).
 MOD_RES 257 257 Phosphoserine (By similarity).
 MOD_RES 266 266 Phosphoserine (By similarity).
 MOD_RES 293 293 Phosphoserine (By similarity).
 MOD_RES 476 476 Phosphoserine; by autocatalysis.  

Keyword

 Apoptosis; ATP-binding; Cell cycle; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 593 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000095; PAK_box_Rho-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom. 

Pfam

 PF00786; PBD
 PF00069; Pkinase 

SMART

 SM00285; PBD
 SM00220; S_TKc 

PROSITE

 PS50108; CRIB
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS