UniProt Accession

 SYY_ECOLI; P0AGJ9; P00951 

Genbank Protein ID

 J01719; U00096; AP009048; M92351 

Genbank Nucleotide ID

 AAA24707.1; AAC74709.1; BAA15398.1; AAA24710.1 

Protein Name

 Tyrosine--tRNA ligase 

Protein Synonyms/Alias

 Tyrosyl-tRNA synthetase; TyrRS 

Gene Name

 tyrS 

Gene Synonyms/Alias

 b1637; JW1629 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
8	MASSNLIKQLQERGL	acetylation	[1]
67	RFQQAGHKPVALVGG	acetylation	[2]
85	LIGDPSFKAAERKLN	acetylation	[1, 2]
103	TVQEWVDKIRKQVAP	acetylation	[2]
144	TFLRDIGKHFSVNQM	acetylation	[1, 2, 3]
154	SVNQMINKEAVKQRL	acetylation	[2]
230	LTVPLITKADGTKFG	acetylation	[2]
235	ITKADGTKFGKTEGG	acetylation	[2]
238	ADGTKFGKTEGGAVW	acetylation	[2]
249	GAVWLDPKKTSPYKF	acetylation	[2]
250	AVWLDPKKTSPYKFY	acetylation	[2]
255	PKKTSPYKFYQFWIN	acetylation	[2]
377	PSRGQARKTIASNAI	acetylation	[2]
390	AITINGEKQSDPEYF	acetylation	[2]
399	SDPEYFFKEEDRLFG	acetylation	[2]
416	TLLRRGKKNYCLICW	acetylation	[1]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). 

Sequence Annotation

 DOMAIN 357 414 S4 RNA-binding.
 MOTIF 42 51 "HIGH" region.
 MOTIF 235 239 "KMSKS" region.
 BINDING 37 37 Tyrosine.
 BINDING 175 175 Tyrosine.
 BINDING 179 179 Tyrosine.
 BINDING 198 198 Tyr-AMP intermediate adenyl group; via
 BINDING 200 200 Tyr-AMP intermediate adenyl group.
 BINDING 228 228 Tyr-AMP intermediate adenyl group; via
 BINDING 238 238 ATP (By similarity).
 MOD_RES 144 144 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 424 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:HAMAP.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:HAMAP. 

Interpro

 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002942; S4_RNA-bd.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type.
 IPR024107; Tyr-tRNA-ligase_bac_1. 

Pfam

 PF01479; S4
 PF00579; tRNA-synt_1b 

SMART

 SM00363; S4 

PROSITE

 PS00178; AA_TRNA_LIGASE_I
 PS50889; S4 

PRINTS

 PR01040; TRNASYNTHTYR.