CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021289
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar protein sorting-associated protein 16 homolog 
Protein Synonyms/Alias
 hVPS16 
Gene Name
 VPS16 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58RNPWRKEKAASVRPVubiquitination[1]
163SANVGDLKLRRMPEVubiquitination[1, 2, 3, 4, 5]
354ALLLEAQKEYEKESQubiquitination[1]
362EYEKESQKADEYLREubiquitination[1, 3]
397EHQPDMQKSLLRAASubiquitination[1]
492LAHWACYKVQQKDVSubiquitination[1]
496ACYKVQQKDVSDEDVubiquitination[1, 3]
510VARAINQKLGDTPGVubiquitination[1, 3, 6, 7]
537GRTELAIKLLEYEPRubiquitination[1, 3]
554EQVPLLLKMKRSKLAubiquitination[1]
617HQELETLKDLYNQDDubiquitination[1]
660TAADAFYKAKNEFAAubiquitination[1, 7]
662ADAFYKAKNEFAAKAubiquitination[1]
668AKNEFAAKATEDQMRubiquitination[1, 3]
745EDWEELEKFSKSKKSubiquitination[1]
750LEKFSKSKKSPIGYLubiquitination[1]
784SRVGPEQKVKALLLVubiquitination[1]
828TDGATADKIQRARAQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. 
Sequence Annotation
 MOD_RES 4 4 Nitrated tyrosine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Endosome; Lysosome; Membrane; Nitration; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 839 AA 
Protein Sequence
MDCYTANWNP LGDSAFYRKY ELYSMDWDLK EELRDCLVAA APYGGPIALL RNPWRKEKAA 60
SVRPVLDIYS ASGMPLASLL WKSGPVVSLG WSAEEELLCV QEDGAVLVYG LHGDFRRHFS 120
MGNEVLQNRV LDARIFHTEF GSGVAILTGA HRFTLSANVG DLKLRRMPEV PGLQSAPSCW 180
TVLCQDRVAH ILLAVGPDLY LLDHAACSAV TPPGLAPGVS SFLQMAVSFT YRHLALFTDT 240
GYIWMGTASL KEKLCEFNCN IRAPPKQMVW CSRPRSKERA VVVAWERRLM VVGDAPESIQ 300
FVLDEDSYLV PELDGVRIFS RSTHEFLHEV PAASEEIFKI ASMAPGALLL EAQKEYEKES 360
QKADEYLREI QELGQLTQAV QQCIEAAGHE HQPDMQKSLL RAASFGKCFL DRFPPDSFVH 420
MCQDLRVLNA VRDYHIGIPL TYSQYKQLTI QVLLDRLVLR RLYPLAIQIC EYLRLPEVQG 480
VSRILAHWAC YKVQQKDVSD EDVARAINQK LGDTPGVSYS DIAARAYGCG RTELAIKLLE 540
YEPRSGEQVP LLLKMKRSKL ALSKAIESGD TDLVFTVLLH LKNELNRGDF FMTLRNQPMA 600
LSLYRQFCKH QELETLKDLY NQDDNHQELG SFHIRASYAA EERIEGRVAA LQTAADAFYK 660
AKNEFAAKAT EDQMRLLRLQ RRLEDELGGQ FLDLSLHDTV TTLILGGHNK RAEQLARDFR 720
IPDKRLWWLK LTALADLEDW EELEKFSKSK KSPIGYLPFV EICMKQHNKY EAKKYASRVG 780
PEQKVKALLL VGDVAQAADV AIEHRNEAEL SLVLSHCTGA TDGATADKIQ RARAQAQKK 839 
Gene Ontology
 GO:0005884; C:actin filament; IEA:Compara.
 GO:0030424; C:axon; IEA:Compara.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0030897; C:HOPS complex; IDA:UniProtKB.
 GO:0005770; C:late endosome; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0055037; C:recycling endosome; IDA:UniProtKB.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR016534; VPS16.
 IPR006925; Vps16_C.
 IPR006926; Vps16_N. 
Pfam
 PF04840; Vps16_C
 PF04841; Vps16_N 
SMART
  
PROSITE
  
PRINTS