CPLM-009053

Genbank Nucleotide ID

Peroxisomal biogenesis factor 3

Protein Synonyms/Alias

Peroxin-3; Peroxisomal assembly protein PEX3

Homo sapiens (Human)

Position	Peptide	Type	References
100	LKNRPSNKLEIWEDL	ubiquitination	[1, 2]
108	LEIWEDLKIISFTRS	ubiquitination	[3]
197	VLGSVSLKHSLSLLD	ubiquitination	[4]
208	SLLDLEQKLKEIRNL	ubiquitination	[3, 4, 5]
220	RNLVEQHKSSSWINK	ubiquitination	[6]
262	PRDITTIKLLNETRD	ubiquitination	[1, 2, 4, 5, 6, 7, 8, 9]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Involved in peroxisome biosynthesis and integrity. Assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, is necessary for the import of peroxisomal membrane proteins in the peroxisomes.

REGION 1 45 Targeting to peroxisomes.
REGION 120 136 Interaction with PEX19.

3D-structure; Complete proteome; Disease mutation; Membrane; Peroxisome; Peroxisome biogenesis; Peroxisome biogenesis disorder; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Zellweger syndrome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IEA:Compara.
GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
GO:0005779; C:integral to peroxisomal membrane; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:HPA.
GO:0043234; C:protein complex; IDA:UniProtKB.
GO:0032994; C:protein-lipid complex; IDA:UniProtKB.
GO:0008289; F:lipid binding; IDA:UniProtKB.
GO:0046983; F:protein dimerization activity; IDA:UniProtKB.
GO:0016557; P:peroxisome membrane biogenesis; IEA:Compara.
GO:0045046; P:protein import into peroxisome membrane; IMP:UniProtKB.

IPR006966; Peroxin-3.