CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015780
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 
Protein Synonyms/Alias
 DEAD/H box 32; DEAD/H helicase-like protein 1; DHLP1; DEAH box protein 32; HuDDX32 
Gene Name
 DHX32 
Gene Synonyms/Alias
 DDX32 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66REDLPIWKEKYSFMEubiquitination[1]
224SSPHLISKLNSYYGNubiquitination[1, 2, 3]
238NVPVIEVKNKHPVEVubiquitination[1]
434TSMVLFMKRIDIAGLubiquitination[2, 3]
569SSEYCVEKWCRDYFLubiquitination[1, 2]
596AELLEIIKRIELPYAubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
 DOMAIN 72 238 Helicase ATP-binding.
 NP_BIND 85 92 ATP (By similarity).
 MOTIF 185 188 DEAH box.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Helicase; Hydrolase; Mitochondrion; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 743 AA 
Protein Sequence
MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS RYYKLLKERE 60
DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA EYCLSIHYQH GGVICTQVHK 120
QTVVQLALRV ADEMDVNIGH EVGYVIPFEN CCTNETILRY CTDDMLQREM MSNPFLGSYG 180
VIILDDIHER SIATDVLLGL LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK 240
HPVEVVYLSE AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQDIEKVCE TVYQGSNLNP 300
DLGELVVVPL YPKEKCSLFK PLDETEKRCQ VYQRRVVLTT SSGEFLIWSN SVRFVIDVGV 360
ERRKVYNPRI RANSLVMQPI SQSQAEIRKQ ILGSSSSGKF FCLYTEEFAS KDMTPLKPAE 420
MQEANLTSMV LFMKRIDIAG LGHCDFMNRP APESLMQALE DLDYLAALDN DGNLSEFGII 480
MSEFPLDPQL SKSILASCEF DCVDEVLTIA AMVTAPNCFS HVPHGAEEAA LTCWKTFLHP 540
EGDHFTLISI YKAYQDTTLN SSSEYCVEKW CRDYFLNCSA LRMADVIRAE LLEIIKRIEL 600
PYAEPAFGSK ENTLNIKKAL LSGYFMQIAR DVDGSGNYLM LTHKQVAQLH PLSGYSITKK 660
MPEWVLFHKF SISENNYIRI TSEISPELFM QLVPQYYFSN LPPSESKDIL QQVVDHLSPV 720
STMNKEQQMC ETCPETEQRC TLQ 743 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW. 
Interpro
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR027417; P-loop_NTPase. 
Pfam
 PF04408; HA2
 PF07717; OB_NTP_bind 
SMART
 SM00847; HA2 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS