CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019048
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger with UFM1-specific peptidase domain protein 
Protein Synonyms/Alias
  
Gene Name
 ZUFSP 
Gene Synonyms/Alias
 C6orf113 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
76QYGTSDNKKDNTLQCubiquitination[1, 2, 3, 4, 5]
77YGTSDNKKDNTLQCGubiquitination[2, 3]
109NSAQNLTKDSTLKHEubiquitination[1, 3]
114LTKDSTLKHEGFYSEubiquitination[1, 3, 5]
135KFLKSREKQSSLTEIubiquitination[3]
174EDMETHVKTKHANLLubiquitination[1]
247LQQEEDRKRRSEESRubiquitination[3]
262QEIEEFQKLQRQYGLacetylation[5, 6]
262QEIEEFQKLQRQYGLubiquitination[1, 2, 3, 4, 5]
276LDNSGGYKQQQLRNMubiquitination[1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12]
301PSEFHRRKADMMESLubiquitination[1, 3, 5]
316ALGFDDGKTKTSGIIubiquitination[3, 5]
318GFDDGKTKTSGIIEAubiquitination[1, 3, 4, 5]
356FHSSLGDKGWGCGYRubiquitination[2, 3, 7, 12]
402SMIEDAWKEGFDPQGubiquitination[1]
447CHIVDFHKSTGPLGTubiquitination[1]
481PKVVCTSKPPIYLQHubiquitination[3]
502VIGIEEKKNRTLCLLubiquitination[3]
522CPSREMQKLLKQDIEubiquitination[3, 5]
525REMQKLLKQDIEASSubiquitination[1, 2, 3, 5, 7, 10, 12]
534DIEASSLKQLRKSMGubiquitination[3, 5, 7, 12]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [11] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [12] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 ZN_FING 2 24 C2H2-type 1.
 ZN_FING 154 177 C2H2-type 2.
 ZN_FING 193 215 C2H2-type 3.
 MOD_RES 262 262 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Metal-binding; Polymorphism; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 578 AA 
Protein Sequence
MLSCNICGET VTSEPDMKAH LIVHMESEII CPFCKLSGVN YDEMCFHIET AHFEQNTLER 60
NFERINTVQY GTSDNKKDNT LQCGMEVNSS ILSGCASNHP KNSAQNLTKD STLKHEGFYS 120
ENLTESRKFL KSREKQSSLT EIKGSVYETT YSPPECPFCG KIEEHSEDME THVKTKHANL 180
LDIPLEDCDQ PLYDCPMCGL ICTNYHILQE HVDLHLEENS FQQGMDRVQC SGDLQLAHQL 240
QQEEDRKRRS EESRQEIEEF QKLQRQYGLD NSGGYKQQQL RNMEIEVNRG RMPPSEFHRR 300
KADMMESLAL GFDDGKTKTS GIIEALHRYY QNAATDVRRV WLSSVVDHFH SSLGDKGWGC 360
GYRNFQMLLS SLLQNDAYND CLKGMLIPCI PKIQSMIEDA WKEGFDPQGA SQLNNRLQGT 420
KAWIGACEVY ILLTSLRVKC HIVDFHKSTG PLGTHPRLFE WILNYYSSEG EGSPKVVCTS 480
KPPIYLQHQG HSRTVIGIEE KKNRTLCLLI LDPGCPSREM QKLLKQDIEA SSLKQLRKSM 540
GNLKHKQYQI LAVEGALSLE EKLARRQASQ VFTAEKIP 578 
Gene Ontology
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR012462; Peptidase_C78_UfSP1/2.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF07910; Peptidase_C78 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS