UniProt Accession

 BAP1_HUMAN; Q92560; A8K993; Q6LEM0; Q7Z5E8 

Genbank Protein ID

 AF045581; D88812; D87462; AK292608; CH471055; BC001596; AY130008 

Genbank Nucleotide ID

 AAC15970.1; BAB46921.1; BAA13401.2; BAF85297.1; EAW65220.1; AAH01596.1; AAN05092.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase BAP1 

Protein Synonyms/Alias

 BRCA1-associated protein 1; Cerebral protein 6 

Gene Name

 BAP1 

Gene Synonyms/Alias

 KIAA0272; hucep-6 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
127	KGFSPESKGYAIGNA	ubiquitination	[1]
139	GNAPELAKAHNSHAR	ubiquitination	[1, 2, 3]
155	EPRHLPEKQNGLSAV	ubiquitination	[1]
275	PELIQTHKSQESQLP	ubiquitination	[1]
630	SGEKYSPKELLALLK	ubiquitination	[1, 2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor. 

Sequence Annotation

 REGION 596 721 Interaction with BRCA1.
 MOTIF 363 366 HBM-like motif.
 MOTIF 717 722 Nuclear localization signal.
 ACT_SITE 91 91 Nucleophile (Probable).
 ACT_SITE 169 169 Proton donor (By similarity).  

Keyword

 Chromatin regulator; Coiled coil; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 729 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0035517; C:PR-DUB complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IMP:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
 GO:0035522; P:monoubiquitinated histone H2A deubiquitination; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0071108; P:protein K48-linked deubiquitination; IMP:UniProtKB.
 GO:0051726; P:regulation of cell cycle; IMP:UniProtKB.
 GO:0001558; P:regulation of cell growth; IMP:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR001578; Peptidase_C12. 

Pfam

 PF01088; Peptidase_C12 

SMART

  

PROSITE

 PS00140; UCH_1 

PRINTS

 PR00707; UBCTHYDRLASE.