UniProt Accession

 DUS3_HUMAN; P51452; D3DX45; Q5U0J1 

Genbank Protein ID

 L05147; BT019522; CH471178; CH471178; BC002682 

Genbank Nucleotide ID

 AAA35777.1; AAV38329.1; EAW51667.1; EAW51668.1; AAH02682.1 

Protein Name

 Dual specificity protein phosphatase 3 

Protein Synonyms/Alias

 Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR 

Gene Name

 DUSP3 

Gene Synonyms/Alias

 VHR 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	SVAQDIPKLQKLGIT	acetylation	[1]
50	SVAQDIPKLQKLGIT	ubiquitination	[2]
53	QDIPKLQKLGITHVL	ubiquitination	[3, 4]
116	IDQALAQKNGRVLVH	ubiquitination	[3, 4, 5]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. 

Sequence Annotation

 DOMAIN 99 169 Tyrosine-protein phosphatase.
 ACT_SITE 124 124 Phosphocysteine intermediate.  

Keyword

 3D-structure; Complete proteome; Hydrolase; Nucleus; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 185 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0001772; C:immunological synapse; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0033549; F:MAP kinase phosphatase activity; IMP:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; IDA:UniProtKB.
 GO:0008138; F:protein tyrosine/serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IDA:UniProtKB.
 GO:0046329; P:negative regulation of JNK cascade; IDA:UniProtKB.
 GO:0050868; P:negative regulation of T cell activation; IDA:UniProtKB.
 GO:0050860; P:negative regulation of T cell receptor signaling pathway; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR020417; Atypical_DUSP.
 IPR020405; Atypical_DUSP_famA.
 IPR000340; Dual-sp_phosphatase_cat-dom.
 IPR020422; Dual-sp_phosphatase_subgr_cat.
 IPR024950; DUSP.
 IPR000387; Tyr/Dual-sp_Pase.
 IPR016130; Tyr_Pase_AS. 

Pfam

 PF00782; DSPc 

SMART

 SM00195; DSPc 

PROSITE

 PS00383; TYR_PHOSPHATASE_1
 PS50056; TYR_PHOSPHATASE_2
 PS50054; TYR_PHOSPHATASE_DUAL 

PRINTS

 PR01908; ADSPHPHTASE.
 PR01909; ADSPHPHTASEA.