UniProt Accession

 RAP2B_HUMAN; P61225; P17964; Q96EG5; Q9CXG0 

Genbank Protein ID

 X52987; AF493915; BC012362 

Genbank Nucleotide ID

 CAA37178.1; AAM12629.1; AAH12362.1 

Protein Name

 Ras-related protein Rap-2b 

Protein Synonyms/Alias

  

Gene Name

 RAP2B 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MREYKVVVLGSG	ubiquitination	[1]
31	VTGSFIEKYDPTIED	ubiquitination	[2, 3, 4]
117	PMILVGNKVDLEGER	ubiquitination	[4]
132	EVSYGEGKALAEEWS	ubiquitination	[5]
150	METSAKNKASVDELF	ubiquitination	[2, 4]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. 

Sequence Annotation

 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 116 119 GTP (By similarity).
 MOTIF 32 40 Effector region (Probable).
 MOD_RES 180 180 Cysteine methyl ester (Probable).
 LIPID 180 180 S-geranylgeranyl cysteine.  

Keyword

 Complete proteome; Endosome; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Palmitate; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 183 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
 GO:0005525; F:GTP binding; TAS:ProtInc.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0030336; P:negative regulation of cell migration; IEA:Compara.
 GO:0031954; P:positive regulation of protein autophosphorylation; IEA:Compara.
 GO:0032486; P:Rap protein signal transduction; ISS:UniProtKB.
 GO:0061097; P:regulation of protein tyrosine kinase activity; ISS:UniProtKB. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR020849; Small_GTPase_Ras. 

Pfam

 PF00071; Ras 

SMART

 SM00173; RAS 

PROSITE

 PS51421; RAS 

PRINTS

 PR00449; RASTRNSFRMNG.