CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019843
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DHX58 
Protein Synonyms/Alias
 Probable ATP-dependent helicase LGP2; Protein D11Lgp2; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor Lgp2; RLR 
Gene Name
 Dhx58 
Gene Synonyms/Alias
 D11lgp2e; Lgp2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
153LSRYLEQKLKKAEPLubiquitination[1]
335LKLFDDHKNVLGQLAubiquitination[1]
350ARGPENPKLEMLERIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. 
Sequence Annotation
 DOMAIN 11 188 Helicase ATP-binding.
 DOMAIN 353 514 Helicase C-terminal.
 NP_BIND 24 31 ATP (Potential).
 REGION 476 678 Repressor domain.
 REGION 572 655 RNA-binding (By similarity).
 MOTIF 131 134 DECH box.
 METAL 556 556 Zinc (By similarity).
 METAL 559 559 Zinc (By similarity).
 METAL 612 612 Zinc (By similarity).
 METAL 615 615 Zinc (By similarity).  
Keyword
 Antiviral defense; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Helicase; Hydrolase; Immunity; Innate immunity; Metal-binding; Nucleotide-binding; Reference proteome; RNA-binding; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 678 AA 
Protein Sequence
MELRPYQWEV ILPALEGKNI IIWLPTGAGK TRAAAFVAKR HLETVDRGKV VVLVNRVHLV 60
SQHAEEFRRM LDKHWTVTTL SGDMGSRAGF GLMARSHDLL ICTAELLQLA LNSSEEDEHV 120
ELREFSLIVV DECHHTHKDT VYNTILSRYL EQKLKKAEPL PQVLGLTASP GTGGATKLQG 180
AIDHILQLCA NLDTCHIMSP KNCYSQLLMH NPKPCKQYDL CQRRAQDPFG DLIKKLMNQI 240
HQQLEMPDLK QQFGTQMYEQ QVVQLCKDAA EAGLQEQRVY ALHLRRYNDA LFIHDTVRAR 300
DALDMLQDFY DRERTTKTQM VRAESWLLKL FDDHKNVLGQ LAARGPENPK LEMLERILLK 360
QFGSPGHTRG IIFTRTRQTA SSLLLWLRQQ PCLQTVGIKP QMLIGAGNTS QSTHMTQKDQ 420
QEVIQEFRDG ILSLLVATSV AEEGLDIAQC NVVVRYGLLT NEISMVQARG RARAGQSVYS 480
FLATEGSREM KRELTNEALE VLMEKAVAAV QKMDPDEFKA KIRDLQQASL VKRAARAAHR 540
EIQQGQFLPE HVQLLCINCM VAVGYGSDLR KVEGTHHVNV NPNFSVYYTT SQNPVVINKV 600
FKDWRPGGTI RCSNCGEVWG FQMIYKSVTL PVLKIGSILL ETPRGKIQAK KWSRVPFSIP 660
VFDILQDCTQ SLSELSLD 678 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003725; F:double-stranded RNA binding; ISS:UniProtKB.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0003727; F:single-stranded RNA binding; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; ISS:UniProtKB.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0045824; P:negative regulation of innate immune response; IMP:UniProtKB.
 GO:0039534; P:negative regulation of MDA-5 signaling pathway; ISS:UniProtKB.
 GO:0039536; P:negative regulation of RIG-I signaling pathway; IMP:UniProtKB.
 GO:0032480; P:negative regulation of type I interferon production; IMP:UniProtKB.
 GO:1900245; P:positive regulation of MDA-5 signaling pathway; IMP:UniProtKB.
 GO:1900246; P:positive regulation of RIG-I signaling pathway; IMP:UniProtKB.
 GO:0032481; P:positive regulation of type I interferon production; IMP:UniProtKB.
 GO:0009615; P:response to virus; IMP:UniProtKB. 
Interpro
 IPR006935; Helicase/UvrB_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR021673; RIG-I_C-RD. 
Pfam
 PF00271; Helicase_C
 PF04851; ResIII
 PF11648; RIG-I_C-RD 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS