UniProt Accession

 AREG_HUMAN; P15514 

Genbank Protein ID

 M30704; M30702; M30698; M30699; M30700; M30701; AY442340; BC009799 

Genbank Nucleotide ID

 AAA51781.1; AAA51773.1; AAA51773.1; AAA51773.1; AAA51773.1; AAA51773.1; AAR05438.1; AAH09799.1 

Protein Name

 Amphiregulin 

Protein Synonyms/Alias

 AR; Colorectum cell-derived growth factor; CRDGF 

Gene Name

 AREG; AREGB 

Gene Synonyms/Alias

 SDGF 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
109	VRVEQVVKPPQNKTE	ubiquitination	[1]
187	RCGEKSMKTHSMIDS	ubiquitination	[2]
230	LRRQYVRKYEGEAEE	ubiquitination	[1, 2]
240	GEAEERKKLRQENGN	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. 

Sequence Annotation

 DOMAIN 142 182 EGF-like.
 CARBOHYD 30 30 N-linked (GlcNAc...) (Potential).
 CARBOHYD 113 113 N-linked (GlcNAc...) (Potential).
 CARBOHYD 119 119 N-linked (GlcNAc...).
 DISULFID 146 159 By similarity.
 DISULFID 154 170 By similarity.
 DISULFID 172 181 By similarity.  

Keyword

 3D-structure; Complete proteome; Cytokine; Direct protein sequencing; Disulfide bond; EGF-like domain; Glycoprotein; Growth factor; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 252 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:BHF-UCL.
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0008083; F:growth factor activity; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; IDA:BHF-UCL.
 GO:0007186; P:G-protein coupled receptor signaling pathway; IDA:BHF-UCL.
 GO:0045740; P:positive regulation of DNA replication; IMP:BHF-UCL. 

Interpro

 IPR000742; EG-like_dom.
 IPR013032; EGF-like_CS.
 IPR015497; EGF_rcpt_ligand. 

Pfam

  

SMART

 SM00181; EGF 

PROSITE

 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3 

PRINTS