UniProt Accession

 DRG1_HUMAN; Q9Y295; B2RDS8; Q6FGP8; Q8WW69; Q9UGF2 

Genbank Protein ID

 AF078103; AJ005940; CR542059; CR456488; BT007237; AK315659; AL096701; AL096702; AL096702; AL096701; CH471095; BC019285; BC020803 

Genbank Nucleotide ID

 AAD12240.1; CAA06775.1; CAG46856.1; CAG30374.1; AAP35901.1; BAG38025.1; CAI12876.1; CAI12876.1; CAH71744.1; CAH71744.1; EAW59976.1; AAH19285.1; AAH20803.1 

Protein Name

 Developmentally-regulated GTP-binding protein 1 

Protein Synonyms/Alias

 DRG-1; Neural precursor cell expressed developmentally down-regulated protein 3; NEDD-3 

Gene Name

 DRG1 

Gene Synonyms/Alias

 NEDD3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
7	*MSSTLAKIAEIEAE	ubiquitination	[1]
32	AHHLGLLKARLAKLR	ubiquitination	[1]
46	RRELITPKGGGGGGP	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
61	GEGFDVAKTGDARIG	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
112	VIRYKGAKIQLLDLP	ubiquitination	[1, 2, 3, 4, 6, 7]
126	PGIIEGAKDGKGRGR	ubiquitination	[1, 3, 4, 6, 7, 8, 9, 10]
129	IEGAKDGKGRGRQVI	ubiquitination	[1, 3, 4]
159	LKPLGHKKIIENELE	ubiquitination	[1, 2, 3, 4, 7, 8, 10, 11, 12]
175	FGIRLNSKPPNIGFK	ubiquitination	[3, 4, 6]
182	KPPNIGFKKKDKGGI	ubiquitination	[3, 4]
186	IGFKKKDKGGINLTA	ubiquitination	[1, 4, 8]
206	ELDAETVKSILAEYK	ubiquitination	[1, 4]
213	KSILAEYKIHNADVT	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 12]
263	EELDIIYKVPHCVPI	ubiquitination	[4]
290	EKIWDYLKLVRIYTK	ubiquitination	[1, 2, 3, 5, 7]
324	TVEDFCMKIHKNLIK	ubiquitination	[1]
331	KIHKNLIKEFKYALV	ubiquitination	[1, 2, 7]
349	SVKHNPQKVGKDHTL	ubiquitination	[3]
352	HNPQKVGKDHTLEDE	ubiquitination	[1, 3, 4, 8]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [11] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [12] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Critical regulator of cell growth under specific conditions. Implicated in differentiation and cell cycle arrest. 

Sequence Annotation

 DOMAIN 76 169 G.
 NP_BIND 71 78 GTP (By similarity).
 NP_BIND 117 121 GTP (By similarity).
 NP_BIND 248 251 GTP (By similarity).
 REGION 1 16 Required for interaction with STK16.
 MOD_RES 100 100 Phosphothreonine; by STK16.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 367 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005844; C:polysome; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0008134; F:transcription factor binding; TAS:ProtInc.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; TAS:ProtInc. 

Interpro

 IPR006074; GTP1-OBG_CS.
 IPR006073; GTP_binding_domain.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR004095; TGS.
 IPR012676; TGS-like. 

Pfam

 PF01926; MMR_HSR1
 PF02824; TGS 

SMART

  

PROSITE

 PS00905; GTP1_OBG 

PRINTS

 PR00326; GTP1OBG.