CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008231
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 5-formyltetrahydrofolate cyclo-ligase 
Protein Synonyms/Alias
 5,10-methenyl-tetrahydrofolate synthetase; MTHFS; Methenyl-THF synthetase 
Gene Name
 MTHFS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10AAAVSSAKRSLRGELubiquitination[1, 2]
39QSRVLSQKVIAHSEYubiquitination[2]
76KDIFQRGKICFIPRYubiquitination[2]
150GNRLGRGKGYYDAYLubiquitination[2]
158GYYDAYLKRCLQHQEubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. 
Sequence Annotation
 NP_BIND 10 14 ATP.
 NP_BIND 147 154 ATP.
 REGION 148 152 Substrate binding.
 BINDING 61 61 Substrate.
 BINDING 109 109 Substrate.
 BINDING 189 189 ATP.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Folate-binding; Ligase; Magnesium; Nucleotide-binding; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 203 AA 
Protein Sequence
MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD 60
EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR 120
EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC 180
LQVPVNENDM KVDEVLYEDS STA 203 
Gene Ontology
 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0030272; F:5-formyltetrahydrofolate cyclo-ligase activity; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005542; F:folic acid binding; IEA:UniProtKB-KW.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0015942; P:formate metabolic process; NAS:UniProtKB.
 GO:0046653; P:tetrahydrofolate metabolic process; IDA:UniProtKB. 
Interpro
 IPR002698; FTHF_cligase.
 IPR024185; FTHF_cligase-like. 
Pfam
 PF01812; 5-FTHF_cyc-lig 
SMART
  
PROSITE
  
PRINTS