CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015516
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-conjugating enzyme E2 O 
Protein Synonyms/Alias
 Ubiquitin carrier protein O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O 
Gene Name
 Ube2o 
Gene Synonyms/Alias
 Kiaa1734 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
127QWYPEGVKQHVKETKubiquitination[1]
212LGKVYDLKNQIILKLubiquitination[1]
516KSIPLSIKNLKRKHKubiquitination[1]
588PGDFVVDKRVQSCPDubiquitination[1]
667TEDGALPKEDEPSVGubiquitination[1]
812PKSFRELKEAIKILEubiquitination[1]
816RELKEAIKILESLKNubiquitination[1]
822IKILESLKNMTVEQLubiquitination[1]
843SPTVEPEKPTREKKFubiquitination[1]
849EKPTREKKFLDDIKKubiquitination[1]
863KLQENLKKTLDNVAIubiquitination[1]
940PSNHSFKKIEFQPPEubiquitination[1]
987DLFSALIKGPTRTPYubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). 
Sequence Annotation
 ACT_SITE 1037 1037 Glycyl thioester intermediate (By
 MOD_RES 82 82 Phosphoserine.
 MOD_RES 84 84 Phosphoserine.
 MOD_RES 394 394 Phosphoserine (By similarity).
 MOD_RES 436 436 Phosphoserine (By similarity).
 MOD_RES 833 833 Phosphoserine.
 MOD_RES 836 836 Phosphoserine.
 MOD_RES 893 893 Phosphoserine.  
Keyword
 ATP-binding; Coiled coil; Complete proteome; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1288 AA 
Protein Sequence
MADPAAPAPA QAQAAAAPTP AAAPAAAAPP PAPATDSASG PSSDSGPEAG SQRLLFSHDL 60
VSGRYRGSVH FGLVRLIHGE DSDSEGDDDG RGSSGCSEAG GAGHEEGRAS PLRRGYVRVQ 120
WYPEGVKQHV KETKLKLEDR SVVPRDVVRH MRSTDSQCGT VIDVNIDCAV KLIGTNCIIY 180
PVNSKDLQHI WPFMYGDYIA YDCWLGKVYD LKNQIILKLS NGARCSMNTE DGAKLYDVCP 240
HVSDSGLFFD DSYGFYPGQV LIGPAKIFSS VQWLSGVKPV LSTKSKFRVV VEEVQVVELK 300
VTWITKSFCP GGTDSVSPPP SIITQENLGR VKRLGCFDHA QRQLGERCLY VFPAKVEPAK 360
IAWECPEKNC AQGEGSMAKK VKRLLKKQVV RIMSCTPDTQ CPRDHSMEDP DKKGEARAGS 420
EIGSASPEEQ PDGSASPVEM QDEGSEELQE TCEPLPPFLL KEGGDDGLHS AEQDADDEAA 480
DDTDDTSSVT SSASSTTSSQ SGSGTGRKKS IPLSIKNLKR KHKRKKNKVT RDFKPGDRVA 540
VEVVTTMTSA DVMWQDGSVE CNIRSNDLFP VHHLDNNEFC PGDFVVDKRV QSCPDPAVYG 600
VVQSGDHVGR TCMVKWFKLR PSGDDVELIG EEEDVSVYDI ADHPDFRFRT TDIVIRIGNT 660
EDGALPKEDE PSVGQVARVD VSSKVEVVWA DNSKTIILPQ HLYNIESEIE ESDYDSVEGS 720
SSGASSDEWE DDSDSWETDN GLVDDEHPKI EELAAILPAE QPTAPEEDKG VVISEEAATA 780
AIQGAVAMAA PVAGLMEKAG KDGPPKSFRE LKEAIKILES LKNMTVEQLL TGSPTSPTVE 840
PEKPTREKKF LDDIKKLQEN LKKTLDNVAI AEEEKMEAVP DTERKEEKPE VQSPVKAEWP 900
SETPVLCQQC GGRPGVTFTS AKGEVFSVLE FAPSNHSFKK IEFQPPEAKK FFSTVRKEMA 960
LLATSLPDGI MVKTFEDRMD LFSALIKGPT RTPYEDGLYL FDIQLPNIYP AVPPHFCYLS 1020
QCSGRLNPNL YDNGKVCVSL LGTWIGKGTE RWTSKSSLLQ VLISIQGLIL VNEPYYNEAG 1080
FDSDRGLQEG YENSRCYNEM ALIRVVQSMT QLVRRPPEVF EQEIRQHFSV GGWRLVNRIE 1140
SWLETHAMQE RAQVMPNGAL KDSSSLEPMA AAELSDSGRE EPEDVGMAPG EASQGSDSEG 1200
GAQGPASASR DHTEQTETAP DASAPPSVRP KRRRKSYRSF LPEKSGYPDI GFPLFPLSKG 1260
FIKSIRGVLT QFRAALLEAG MPESTEDK 1288 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC. 
Interpro
 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF00179; UQ_con 
SMART
  
PROSITE
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS