UniProt Accession

 HHIP_HUMAN; Q96QV1; Q6PK09; Q8NCI7; Q9BXK3; Q9H1J4; Q9H7E7 

Genbank Protein ID

 AY009951; AF326471; AF326459; AF326460; AF326462; AF326464; AF326466; AF326468; AF326470; AF326469; AF326467; AF326465; AF326463; AF326461; AY009317; AY358747; AK024645; AK074711; AC098588; BC009298; BC025311 

Genbank Nucleotide ID

 AAG34731.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAK18182.1; AAG35411.1; AAQ89107.1; BAB14945.1; BAC11154.1; AAH09298.1; AAH25311.1 

Protein Name

 Hedgehog-interacting protein 

Protein Synonyms/Alias

 HHIP; HIP 

Gene Name

 HHIP 

Gene Synonyms/Alias

 HIP; UNQ5825/PRO19644 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
93	GLGRLENKIFSVTNN	ubiquitination	[1]
105	TNNTECGKLLEEIKC	ubiquitination	[1]
142	LVLPLLCKDYCKEFF	ubiquitination	[1]
174	FCFYYARKDGGLCFP	ubiquitination	[1]
247	QRLFILEKEGYVKIL	ubiquitination	[1]
252	LEKEGYVKILTPEGE	ubiquitination	[1]
262	TPEGEIFKEPYLDIH	ubiquitination	[1]
270	EPYLDIHKLVQSGIK	ubiquitination	[1]
277	KLVQSGIKGGDERGL	ubiquitination	[1]
329	VEYTVSRKNPHQVDL	ubiquitination	[1]
472	ILQIIKGKDYESEPS	ubiquitination	[1]
528	LQQSPVTKQWQEKPL	ubiquitination	[1]
533	VTKQWQEKPLCLGTS	ubiquitination	[1]
622	GYCTPTGKCCCSPGW	ubiquitination	[1]
659	PNKCLCKKGYLGPQC	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. 

Sequence Annotation

 DOMAIN 607 634 EGF-like 1.
 DOMAIN 635 667 EGF-like 2.
 REGION 376 388 Interaction with SHH zinc binding site.
 METAL 383 383 Zinc; shared with SHH.
 CARBOHYD 99 99 N-linked (GlcNAc...) (Potential).
 CARBOHYD 416 416 N-linked (GlcNAc...) (Potential).
 CARBOHYD 447 447 N-linked (GlcNAc...) (Potential).
 CARBOHYD 459 459 N-linked (GlcNAc...) (Potential).
 DISULFID 216 536
 DISULFID 218 543
 DISULFID 402 624
 DISULFID 435 452
 DISULFID 500 594
 DISULFID 608 617
 DISULFID 612 623
 DISULFID 625 634
 DISULFID 639 649
 DISULFID 643 655
 DISULFID 657 666  

Keyword

 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Disulfide bond; EGF-like domain; Glycoprotein; Membrane; Metal-binding; Polymorphism; Reference proteome; Repeat; Secreted; Signal; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 700 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; ISS:BHF-UCL.
 GO:0016901; F:oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor; IEA:InterPro.
 GO:0048038; F:quinone binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
 GO:0009953; P:dorsal/ventral pattern formation; IEA:Compara.
 GO:0060441; P:epithelial tube branching involved in lung morphogenesis; IEA:Compara.
 GO:0045879; P:negative regulation of smoothened signaling pathway; ISS:BHF-UCL.
 GO:0007405; P:neuroblast proliferation; IEA:Compara.
 GO:0040036; P:regulation of fibroblast growth factor receptor signaling pathway; IEA:Compara.
 GO:0048705; P:skeletal system morphogenesis; ISS:BHF-UCL.
 GO:0007224; P:smoothened signaling pathway; IEA:Compara. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR000742; EG-like_dom.
 IPR013032; EGF-like_CS.
 IPR018143; Folate_rcpt-like.
 IPR012938; Glc/Sorbosone_DH.
 IPR011041; Quinoprot_gluc/sorb_DH. 

Pfam

 PF03024; Folate_rec
 PF07995; GSDH 

SMART

 SM00181; EGF 

PROSITE

 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3 

PRINTS