CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041428
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Diacylglycerol kinase alpha 
Protein Synonyms/Alias
  
Gene Name
 DGKA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130GQHMWRPKRFPRPVYubiquitination[1]
158GLSCNLCKYTVHDQCubiquitination[1]
168VHDQCAMKALPCEVSubiquitination[1, 2, 3]
179CEVSTYAKSRKDIGVubiquitination[1, 3]
182STYAKSRKDIGVQSHubiquitination[1]
272KNSKTSQKTMDDLNLacetylation[3]
272KNSKTSQKTMDDLNLubiquitination[1, 3, 4]
303LLVFVNPKSGGKQGQubiquitination[1, 3]
307VNPKSGGKQGQRVLWubiquitination[1]
330RQVFNLLKDGPEIGLubiquitination[1, 3, 4]
341EIGLRLFKDVPDSRIubiquitination[1, 2, 3]
403YEGQNLAKILKDLEMubiquitination[1]
406QNLAKILKDLEMSKVubiquitination[1, 4]
412LKDLEMSKVVHMDRWubiquitination[1, 3, 4]
462RFHIMREKYPEKFNSubiquitination[1]
466MREKYPEKFNSRMKNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Calcium; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 545 AA 
Protein Sequence
MAKERGLISP SDFAQLQKYM EYSTKKGGRP EDKLEFTFKL YDTDRNGILD SSEVDKIILQ 60
MMRVAEYLDW DVSELRPILQ EMMKEIDYDG SGSVSQAEWV RAGATTVPLL VLLGLEMTLK 120
DDGQHMWRPK RFPRPVYCNL CESSIGLGKQ GLSCNLCKYT VHDQCAMKAL PCEVSTYAKS 180
RKDIGVQSHV WVRGGCESGR CDRCQKKIRI YHSLTGLHCV WCHLEIHDDC LQAVGHECDC 240
GLLRDHILPP SSIYPSVLAS GPDRKNSKTS QKTMDDLNLS TSEALRIDPV PNTHPLLVFV 300
NPKSGGKQGQ RVLWKFQYIL NPRQVFNLLK DGPEIGLRLF KDVPDSRILV CGGDGTVGWI 360
LETIDKANLP VLPPVAVLPL GTGNDLARCL RWGGGYEGQN LAKILKDLEM SKVVHMDRWS 420
VEVIPQQTEE KSDPVPFQII NNYFSIGVDA SIAHRFHIMR EKYPEKFNSR MKNKLWYFEF 480
ATSESIFSTC KKLEESLTVE ICGKPLDLSN LSLEGIAVLN IPSMHGGSNL WGDTRRPHGD 540
IYGIN 545 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004143; F:diacylglycerol kinase activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000756; Diacylglycerol_kin_accessory.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd. 
Pfam
 PF00130; C1_1
 PF00609; DAGK_acc
 PF00781; DAGK_cat
 PF13405; EF_hand_4 
SMART
 SM00109; C1
 SM00045; DAGKa
 SM00046; DAGKc
 SM00054; EFh 
PROSITE
 PS50146; DAGK
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS