UniProt Accession

 RFC5_HUMAN; P40937; A8MZ62; B3KSX8 

Genbank Protein ID

 L07540; AY254323; AK094575; AC131159; BC001866; BC013961 

Genbank Nucleotide ID

 AAB09784.1; AAO63493.1; BAG52890.1; AAH01866.1; AAH13961.1 

Protein Name

 Replication factor C subunit 5 

Protein Synonyms/Alias

 Activator 1 36 kDa subunit; A1 36 kDa subunit; Activator 1 subunit 5; Replication factor C 36 kDa subunit; RF-C 36 kDa subunit; RFC36 

Gene Name

 RFC5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
7	*METSALKQQEQPAA	ubiquitination	[1, 2, 3]
16	QEQPAATKIRNLPWV	ubiquitination	[1, 2, 4]
25	RNLPWVEKYRPQTLN	ubiquitination	[2, 4]
66	YGPPGTGKTSTILAC	ubiquitination	[2]
75	STILACAKQLYKDKE	ubiquitination	[2]
144	ALRRVIEKFTENTRF	ubiquitination	[2, 4]
194	EHVVEEEKVDISEDG	ubiquitination	[2, 4]
203	DISEDGMKALVTLSS	ubiquitination	[4]
270	YRNITELKTLKGLAL	ubiquitination	[2, 4, 5]
273	ITELKTLKGLALHDI	ubiquitination	[2]
304	VRIHLLTKMADIEYR	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. 

Sequence Annotation

 NP_BIND 60 67 ATP (Potential).  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; DNA replication; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 340 AA 

Protein Sequence

Gene Ontology

 GO:0005663; C:DNA replication factor C complex; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0019899; F:enzyme binding; NAS:UniProtKB.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0006297; P:nucleotide-excision repair, DNA gap filling; TAS:Reactome.
 GO:0000722; P:telomere maintenance via recombination; TAS:Reactome.
 GO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR008921; DNA_pol3_clamp-load_cplx_C.
 IPR027417; P-loop_NTPase.
 IPR013748; Rep_factorC_C_dom. 

Pfam

 PF00004; AAA
 PF08542; Rep_fac_C 

SMART

 SM00382; AAA 

PROSITE

  

PRINTS