CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044778
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG binding domain protein 3, isoform CRA_b 
Protein Synonyms/Alias
 Methyl-CpG-binding domain protein 3 
Gene Name
 MBD3 
Gene Synonyms/Alias
 hCG_22692 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17TGKMLMSKMNKSRQRubiquitination[1]
34YDSSNQVKGKPDLNTubiquitination[1]
36SSNQVKGKPDLNTALubiquitination[1, 2]
73KVKSDPQKAVDQPRQubiquitination[1]
85PRQLFWEKKLSGLNAacetylation[2, 3]
85PRQLFWEKKLSGLNAubiquitination[1, 4]
86RQLFWEKKLSGLNAFubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 235 AA 
Protein Sequence
MDLSTFDFRT GKMLMSKMNK SRQRVRYDSS NQVKGKPDLN TALPVRQTAS IFKQPVTKIT 60
NHPSNKVKSD PQKAVDQPRQ LFWEKKLSGL NAFDIAEELV KTMDLPKGLQ GVGPGCTDET 120
LLSAIASALH TSTMPITGQL SAAVEKNPGV WLNTTQPLCK AFMVTDEDIR KQEELVQQVR 180
KRLEEALMAD MLAHVEELAR DGEAPLDKAC AEDDDEEDEE EEEEEPDPDP EMEHV 235 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000792; C:heterochromatin; IEA:Compara.
 GO:0016581; C:NuRD complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0016573; P:histone acetylation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0006346; P:methylation-dependent chromatin silencing; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0009888; P:tissue development; IEA:Compara. 
Interpro
 IPR025884; MeCpG-bd_2/3_C_dom.
 IPR001739; Methyl_CpG_DNA-bd. 
Pfam
 PF14048; MBD_C 
SMART
  
PROSITE
  
PRINTS