CPLM-011563

Genbank Nucleotide ID

Exosome complex exonuclease DIS3

Protein Synonyms/Alias

Chromosome disjunction protein 3; Ribosomal RNA-processing protein 44

RRP44; YOL021C; O2197

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
375	KQRRLLAKDAMIAQR	acetylation	[1]

[1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]

Functional Description

Catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. DIS3 has both 3'-5' exonuclease and endonuclease activities. The exonuclease activity of DIS3 is down- regulated upon association with Exo-9 possibly involving a conformational change in the catalytic domain and threading of the RNA substrate through the complex central channel. Structured substrates can be degraded if they have a 3' single-stranded extension sufficiently long (such as 35 nt poly(A)) to span the proposed complex inner RNA-binding path and to reach the exonuclease site provided by DIS3. Plays a role in mitotic control.

DOMAIN 904 1001 S1 motif.
REGION 1 235 Endoribonuclease.

3D-structure; Complete proteome; Cytoplasm; Endonuclease; Exonuclease; Exosome; Hydrolase; Mitochondrion; Nuclease; Nucleus; Reference proteome; RNA-binding; rRNA processing.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000177; C:cytoplasmic exosome (RNase complex); IDA:SGD.
GO:0005739; C:mitochondrion; IDA:SGD.
GO:0000176; C:nuclear exosome (RNase complex); IDA:SGD.
GO:0005730; C:nucleolus; IDA:SGD.
GO:0000175; F:3'-5'-exoribonuclease activity; IDA:SGD.
GO:0004521; F:endoribonuclease activity; IDA:SGD.
GO:0000049; F:tRNA binding; IDA:SGD.
GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
GO:0070651; P:nonfunctional rRNA decay; IMP:SGD.
GO:0071028; P:nuclear mRNA surveillance; IMP:SGD.
GO:0071039; P:nuclear polyadenylation-dependent CUT catabolic process; IMP:SGD.
GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IC:SGD.
GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:SGD.
GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IDA:SGD.
GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IC:SGD.
GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IC:SGD.
GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IC:SGD.

IPR002716; PIN_dom.
IPR006596; PINc_nuc-bd.
IPR003029; Rbsml_prot_S1_RNA-bd_dom.
IPR022967; RNA-binding_domain_S1.
IPR001900; RNase_II/R.
IPR022966; RNase_II/R_CS.

PF13638; PIN_4
PF00773; RNB

SM00670; PINc
SM00955; RNB
SM00316; S1

PS01175; RIBONUCLEASE_II
PS50126; S1