UniProt Accession

 ENOG_RAT; P07323 

Genbank Protein ID

 M11931; M22770; AF019973; BC060310; X07727; X07728; X07729 

Genbank Nucleotide ID

 AAA41119.1; AAA41725.1; AAB72088.1; AAH60310.1; CAA30556.1; CAA30556.1; CAA30556.1 

Protein Name

 Gamma-enolase 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE 

Gene Name

 Eno2 

Gene Synonyms/Alias

 Eno-2 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
5	***MSIQKIWAREIL	acetylation	[1]
54	LELRDGDKQRYLGKG	acetylation	[1]
60	DKQRYLGKGVLKAVD	acetylation	[1]
193	AEVYHTLKGVIKDKY	acetylation	[1]
197	HTLKGVIKDKYGKDA	acetylation	[1]
199	LKGVIKDKYGKDATN	acetylation	[1]
233	LVKEAIDKAGYTEKM	acetylation	[1]
256	SEFYRDGKYDLDFKS	acetylation	[1]
406	CRSERLAKYNQLMRI	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. 

Sequence Annotation

 REGION 370 373 Substrate binding (By similarity).
 ACT_SITE 210 210 Proton donor (By similarity).
 ACT_SITE 343 343 Proton acceptor (By similarity).
 METAL 245 245 Magnesium (By similarity).
 METAL 293 293 Magnesium (By similarity).
 METAL 318 318 Magnesium (By similarity).
 BINDING 158 158 Substrate (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 293 293 Substrate (By similarity).
 BINDING 318 318 Substrate (By similarity).
 BINDING 394 394 Substrate (By similarity).
 MOD_RES 26 26 Phosphothreonine (By similarity).
 MOD_RES 44 44 Phosphotyrosine (By similarity).
 MOD_RES 80 80 Phosphoserine (By similarity).  

Keyword

 Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 434 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:RGD.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0097060; C:synaptic membrane; IDA:RGD.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD.
 GO:0046982; F:protein heterodimerization activity; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0006094; P:gluconeogenesis; IDA:RGD.
 GO:0006096; P:glycolysis; IDA:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0032355; P:response to estradiol stimulus; IEP:RGD. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.