CPLM-013463

Genbank Nucleotide ID

Protein Synonyms/Alias

Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein

Homo sapiens (Human)

Position	Peptide	Type	References
37	REVEIRIKQIESDRQ	ubiquitination	[1, 2]
109	EAIQTPLKSAKTRKV	ubiquitination	[3]
183	RLEVSMVKPTPGLTP	acetylation	[4]
183	RLEVSMVKPTPGLTP	ubiquitination	[1, 2]
198	RFDSRVFKTPGLRTP	ubiquitination	[1]
225	GSPLADSKEIFLTVP	ubiquitination	[1, 5]
263	PEALGNIKKLSNRLA	ubiquitination	[5]
264	EALGNIKKLSNRLAQ	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

REGION 1 140 Required for interaction with SENP3.
REGION 1 88 Required for centromere localization.
REGION 1 58 Required for interaction with INCENP.
REGION 10 109 Required to form a minimal CPC core
REGION 20 78 Required for interaction with INCENP and
MOD_RES 88 88 Phosphothreonine; by TTK.
MOD_RES 94 94 Phosphothreonine; by TTK.
MOD_RES 106 106 Phosphothreonine.
MOD_RES 110 110 Phosphoserine.
MOD_RES 165 165 Phosphoserine; by AURKB.
MOD_RES 169 169 Phosphothreonine; by TTK.
MOD_RES 189 189 Phosphothreonine.
MOD_RES 204 204 Phosphothreonine.
MOD_RES 219 219 Phosphoserine.
MOD_RES 224 224 Phosphoserine.
MOD_RES 230 230 Phosphothreonine; by TTK.
MOD_RES 238 238 Phosphoserine.
MOD_RES 244 244 Phosphoserine.

3D-structure; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0010369; C:chromocenter; IEA:Compara.
GO:0032133; C:chromosome passenger complex; IPI:UniProtKB.
GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005825; C:half bridge of spindle pole body; IDA:HPA.
GO:0030496; C:midbody; IDA:FlyBase.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0051276; P:chromosome organization; IMP:UniProtKB.
GO:0000278; P:mitotic cell cycle; TAS:Reactome.
GO:0000089; P:mitotic metaphase; IMP:UniProtKB.

IPR018851; Borealin-like_N.
IPR018867; Cell_div_borealin.

PF10512; Borealin
PF10444; Nbl1_Borealin_N