CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017159
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hydroxymethylglutaryl-CoA synthase, cytoplasmic 
Protein Synonyms/Alias
 HMG-CoA synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase 
Gene Name
 Hmgcs1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
46YDGVDAGKYTIGLGQacetylation[1]
46YDGVDAGKYTIGLGQubiquitination[2]
206QHAYDFYKPDMLSEYacetylation[1]
206QHAYDFYKPDMLSEYubiquitination[2]
273PYCKLVQKSLARMFLacetylation[3]
273PYCKLVQKSLARMFLubiquitination[2]
291LNDQNRDKNSIYSGLubiquitination[2]
317YFDRDVEKAFMKASSubiquitination[2]
321DVEKAFMKASSELFNacetylation[3]
321DVEKAFMKASSELFNubiquitination[2]
330SSELFNQKTKASLLVacetylation[3]
330SSELFNQKTKASLLVubiquitination[2]
400TPGSALDKITASLCDubiquitination[2]
409TASLCDLKSRLDSRTacetylation[3]
409TASLCDLKSRLDSRTubiquitination[2]
428DVFAENMKLREDTHHacetylation[3]
428DVFAENMKLREDTHHubiquitination[2]
498EHIPSPAKKVPRLPAubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase (By similarity). 
Sequence Annotation
 ACT_SITE 129 129 Potential.
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 46 46 N6-acetyllysine (By similarity).
 MOD_RES 273 273 N6-acetyllysine (By similarity).
 MOD_RES 495 495 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Lipid biosynthesis; Lipid metabolism; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 520 AA 
Protein Sequence
MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG LGQARMGFCT 60
DREDINSLCL TVVQKLMERH SLSYDCIGRL EVGTETIIDK SKSVKSNLMQ LFEESGNTDI 120
EGIDTTNACY GGTAAVFNAV NWVESSSWDG RYALVVAGDI AIYATGNARP TGGVGAVALL 180
IGPNAPLIFD RGLRGTHMQH AYDFYKPDML SEYPVVDGKL SIQCYLSALD RCYSVYRKKI 240
RAQWQKEGKD KDFTLNDFGF MIFHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA 300
FGDVKLEDTY FDRDVEKAFM KASSELFNQK TKASLLVSNQ NGNMYTSSVY GSLASVLAQY 360
SPQQLAGKRV GVFSYGSGLA ATLYSLKVTQ DATPGSALDK ITASLCDLKS RLDSRTCVAP 420
DVFAENMKLR EDTHHLANYI PQCSIDSLFE GTWYLVRVDE KHRRTYARRP FTNDHSLDEG 480
MGLVHSNTAT EHIPSPAKKV PRLPATSAES ESAVISNGEH 520 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0008144; F:drug binding; IEA:Compara.
 GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:EC.
 GO:0016853; F:isomerase activity; IEA:Compara.
 GO:0043177; F:organic acid binding; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0071397; P:cellular response to cholesterol; IEA:Compara.
 GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
 GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0008584; P:male gonad development; IEA:Compara.
 GO:0001101; P:response to acid; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0055094; P:response to lipoprotein particle stimulus; IEA:Compara.
 GO:0009645; P:response to low light intensity stimulus; IEA:Compara.
 GO:0014074; P:response to purine-containing compound; IEA:Compara.
 GO:0046690; P:response to tellurium ion; IEA:Compara.
 GO:0033197; P:response to vitamin E; IEA:Compara. 
Interpro
 IPR000590; HMG_CoA_synt_AS.
 IPR013746; HMG_CoA_synt_C.
 IPR013528; HMG_CoA_synth_N.
 IPR010122; HMG_CoA_synthase_euk.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr. 
Pfam
 PF08540; HMG_CoA_synt_C
 PF01154; HMG_CoA_synt_N 
SMART
  
PROSITE
 PS01226; HMG_COA_SYNTHASE 
PRINTS