UniProt Accession

 GPDA_RAT; O35077; Q5I0F4 

Genbank Protein ID

 AB002558; BC088396 

Genbank Nucleotide ID

 BAA21763.1; AAH88396.1 

Protein Name

 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic 

Protein Synonyms/Alias

 GPD-C; GPDH-C 

Gene Name

 Gpd1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
49	EEDIGGRKLTEIINT	ubiquitination	[1]
62	NTQHENVKYLPGHKL	acetylation	[2]
106	GKICDQLKGHLKANT	acetylation	[2]
120	TIGISLIKGIDEGPN	acetylation	[2]
282	EAFARTGKSIEQLEK	acetylation	[2]
289	KSIEQLEKEMLNGQK	acetylation	[2]
296	KEMLNGQKLQGPQTA	acetylation	[2]
313	LHSILQHKGLVDKFP	acetylation	[2]
318	QHKGLVDKFPLFTAV	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

  

Sequence Annotation

 NP_BIND 10 15 NAD (By similarity).
 REGION 269 270 Substrate binding (By similarity).
 ACT_SITE 204 204 Proton acceptor (Potential).
 BINDING 41 41 NAD (By similarity).
 BINDING 97 97 NAD (By similarity).
 BINDING 120 120 Substrate (By similarity).
 BINDING 153 153 NAD; via amide nitrogen (By similarity).
 BINDING 269 269 NAD (By similarity).
 BINDING 296 296 NAD; via amide nitrogen (By similarity).
 BINDING 298 298 NAD (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 349 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IDA:RGD.
 GO:0004368; F:glycerol-3-phosphate dehydrogenase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0005975; P:carbohydrate metabolic process; IDA:RGD.
 GO:0071320; P:cellular response to cAMP; IEA:Compara.
 GO:0071356; P:cellular response to tumor necrosis factor; IEA:Compara.
 GO:0006094; P:gluconeogenesis; IEA:Compara.
 GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
 GO:0006072; P:glycerol-3-phosphate metabolic process; IDA:RGD.
 GO:0046486; P:glycerolipid metabolic process; IDA:RGD.
 GO:0006116; P:NADH oxidation; IDA:RGD. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR013328; DH_multihelical.
 IPR006168; G3P_DH_NAD-dep.
 IPR006109; G3P_DH_NAD-dep_C.
 IPR017751; G3P_DH_NAD-dep_euk.
 IPR011128; G3P_DH_NAD-dep_N.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF07479; NAD_Gly3P_dh_C
 PF01210; NAD_Gly3P_dh_N 

SMART

  

PROSITE

 PS00957; NAD_G3PDH 

PRINTS

 PR00077; GPDHDRGNASE.