CPLM-017036

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-017036

UniProt Accession

UBR2_HUMAN; Q8IWV8; O15057; Q4VXK2; Q5TFH6; Q6P2I2; Q6ZUD0

Genbank Protein ID

AY061884; AK125795; AL049843; AL136223; AL391814; AL049843; AL136223; AL391814; AL136223; AL391814; AL136223; AL049843; AL391814; AL136223; AL049843; AL391814; AL391814; AL136223; AL391814; AL136223; AL049843; AL391814; AL049843; AL136223; BC024217; BC064512; AB002347

Genbank Nucleotide ID

AAL32101.1; BAC86295.1; CAI20399.1; CAI20399.1; CAI20399.1; CAI95685.1; CAI95685.1; CAI95685.1; CAI19919.1; CAI19919.1; CAI19920.1; CAI19920.1; CAI19920.1; CAI95666.1; CAI95666.1; CAI95666.1; CAI14737.1; CAI14737.1; CAI14738.1; CAI14738.1; CAI14738.1; CAI95298.1; CAI95298.1; CAI95298.1; AAH24217.1; AAH64512.1; BAA20806.1

Protein Name

E3 ubiquitin-protein ligase UBR2

Protein Synonyms/Alias

N-recognin-2; Ubiquitin-protein ligase E3-alpha-2; Ubiquitin-protein ligase E3-alpha-II

Gene Name

UBR2

Gene Synonyms/Alias

C6orf133; KIAA0349

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
1187	YFDSVQAKEQRRQQR	ubiquitination	[1, 2]
1352	RILSDEDKPLFGPLP	ubiquitination	[1]

Reference

[1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.

Sequence Annotation

ZN_FING 97 168 UBR-type.
ZN_FING 1108 1214 RING-type; atypical.

Keyword

3D-structure; Alternative splicing; Coiled coil; Complete proteome; Ligase; Metal-binding; Nucleus; Polymorphism; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1755 AA

Protein Sequence

MASELEPEVQ AIDRSLLECS AEEIAGKWLQ ATDLTREVYQ HLAHYVPKIY CRGPNPFPQK 60
EDMLAQHVLL GPMEWYLCGE DPAFGFPKLE QANKPSHLCG RVFKVGEPTY SCRDCAVDPT 120
CVLCMECFLG SIHRDHRYRM TTSGGGGFCD CGDTEAWKEG PYCQKHELNT SEIEEEEDPL 180
VHLSEDVIAR TYNIFAITFR YAVEILTWEK ESELPADLEM VEKSDTYYCM LFNDEVHTYE 240
QVIYTLQKAV NCTQKEAIGF ATTVDRDGRR SVRYGDFQYC EQAKSVIVRN TSRQTKPLKV 300
QVMHSSIVAH QNFGLKLLSW LGSIIGYSDG LRRILCQVGL QEGPDGENSS LVDRLMLSDS 360
KLWKGARSVY HQLFMSSLLM DLKYKKLFAV RFAKNYERLQ SDYVTDDHDR EFSVADLSVQ 420
IFTVPSLARM LITEENLMSI IIKTFMDHLR HRDAQGRFQF ERYTALQAFK FRRVQSLILD 480
LKYVLISKPT EWSDELRQKF LEGFDAFLEL LKCMQGMDPI TRQVGQHIEM EPEWEAAFTL 540
QMKLTHVISM MQDWCASDEK VLIEAYKKCL AVLMQCHGGY TDGEQPITLS ICGHSVETIR 600
YCVSQEKVSI HLPVSRLLAG LHVLLSKSEV AYKFPELLPL SELSPPMLIE HPLRCLVLCA 660
QVHAGMWRRN GFSLVNQIYY YHNVKCRREM FDKDVVMLQT GVSMMDPNHF LMIMLSRFEL 720
YQIFSTPDYG KRFSSEITHK DVVQQNNTLI EEMLYLIIML VGERFSPGVG QVNATDEIKR 780
EIIHQLSIKP MAHSELVKSL PEDENKETGM ESVIEAVAHF KKPGLTGRGM YELKPECAKE 840
FNLYFYHFSR AEQSKAEEAQ RKLKRQNRED TALPPPVLPP FCPLFASLVN ILQSDVMLCI 900
MGTILQWAVE HNGYAWSESM LQRVLHLIGM ALQEEKQHLE NVTEEHVVTF TFTQKISKPG 960
EAPKNSPSIL AMLETLQNAP YLEVHKDMIR WILKTFNAVK KMRESSPTSP VAETEGTIME 1020
ESSRDKDKAE RKRKAEIARL RREKIMAQMS EMQRHFIDEN KELFQQTLEL DASTSAVLDH 1080
SPVASDMTLT ALGPAQTQVP EQRQFVTCIL CQEEQEVKVE SRAMVLAAFV QRSTVLSKNR 1140
SKFIQDPEKY DPLFMHPDLS CGTHTSSCGH IMHAHCWQRY FDSVQAKEQR RQQRLRLHTS 1200
YDVENGEFLC PLCECLSNTV IPLLLPPRNI FNNRLNFSDQ PNLTQWIRTI SQQIKALQFL 1260
RKEESTPNNA STKNSENVDE LQLPEGFRPD FRPKIPYSES IKEMLTTFGT ATYKVGLKVH 1320
PNEEDPRVPI MCWGSCAYTI QSIERILSDE DKPLFGPLPC RLDDCLRSLT RFAAAHWTVA 1380
SVSVVQGHFC KLFASLVPND SHEELPCILD IDMFHLLVGL VLAFPALQCQ DFSGISLGTG 1440
DLHIFHLVTM AHIIQILLTS CTEENGMDQE NPPCEEESAV LALYKTLHQY TGSALKEIPS 1500
GWHLWRSVRA GIMPFLKCSA LFFHYLNGVP SPPDIQVPGT SHFEHLCSYL SLPNNLICLF 1560
QENSEIMNSL IESWCRNSEV KRYLEGERDA IRYPRESNKL INLPEDYSSL INQASNFSCP 1620
KSGGDKSRAP TLCLVCGSLL CSQSYCCQTE LEGEDVGACT AHTYSCGSGV GIFLRVRECQ 1680
VLFLAGKTKG CFYSPPYLDD YGETDQGLRR GNPLHLCKER FKKIQKLWHQ HSVTEEIGHA 1740
QEANQTLVGI DWQHL 1755

Gene Ontology

GO:0000785; C:chromatin; IEA:Compara.
GO:0005634; C:nucleus; IDA:HPA.
GO:0005886; C:plasma membrane; IDA:HPA.
GO:0000151; C:ubiquitin ligase complex; IEA:Compara.
GO:0070728; F:leucine binding; IDA:UniProtKB.
GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0071233; P:cellular response to leucine; IDA:UniProtKB.
GO:0006342; P:chromatin silencing; ISS:UniProtKB.
GO:0033522; P:histone H2A ubiquitination; ISS:UniProtKB.
GO:0007141; P:male meiosis I; IEA:Compara.
GO:0032007; P:negative regulation of TOR signaling cascade; IMP:UniProtKB.
GO:0007283; P:spermatogenesis; IEA:Compara.
GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Compara.

Interpro

IPR003769; ClpS_core.
IPR014719; Ribosomal_L7/12_C/ClpS-like.
IPR003126; Znf_N-recognin.
IPR013993; Znf_N-recognin_met.
IPR001841; Znf_RING.

Pfam

PF02617; ClpS
PF02207; zf-UBR

SMART

SM00184; RING
SM00396; ZnF_UBR1

PROSITE

PS00518; ZF_RING_1
PS50089; ZF_RING_2
PS51157; ZF_UBR

PRINTS