CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006164
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 FACT complex subunit SPT16 
Protein Synonyms/Alias
 Cell division control protein 68; Facilitates chromatin transcription complex subunit SPT16; Suppressor of Ty protein 16 
Gene Name
 SPT16 
Gene Synonyms/Alias
 CDC68; SSF1; YGL207W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
211RAVDEELKITNAKLSacetylation[1]
224LSDKIENKIDDVKFLacetylation[1]
229ENKIDDVKFLKQLSPacetylation[1]
382SNFILNVKNDYRKIQacetylation[1]
583NSYKNGSKNEEGEYTubiquitination[2]
608SSGGISKKVEELPYEubiquitination[2]
668ADVVQQDKLIENKTGacetylation[1]
800QEQEERRKRAALDKEubiquitination[3]
806RKRAALDKEFKYFADubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication. 
Sequence Annotation
 MOD_RES 526 526 Phosphoserine.
 MOD_RES 765 765 Phosphoserine.  
Keyword
 3D-structure; Activator; Chromosome; Coiled coil; Complete proteome; DNA damage; DNA repair; DNA replication; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1035 AA 
Protein Sequence
MEELNIDFDV FKKRIELLYS KYNEFEGSPN SLLFVLGSSN AENPYQKTTI LHNWLLSYEF 60
PATLIALVPG KVIIITSSAK AKHLQKAIDL FKDPESKITL ELWQRNNKEP ELNKKLFDDV 120
IALINSAGKT VGIPEKDSYQ GKFMTEWNPV WEAAVKENEF NVIDISLGLS KVWEVKDVNE 180
QAFLSVSSKG SDKFMDLLSN EMVRAVDEEL KITNAKLSDK IENKIDDVKF LKQLSPDLSA 240
LCPPNYKFNF DLLDWTYSPI IQSGKKFDLR VSARSTNDQL YGNGCILASC GIRYNNYCSN 300
ITRTFLIDPS EEMANNYDFL LTLQKEIVTN ILKPGRTPKE VYESVIEYIE KTKPELVPNF 360
TKNIGSLIGL EFRDSNFILN VKNDYRKIQR GDCFNISFGF NNLKDSQSAN NYALQLADTV 420
QIPLDETEPP RFLTNYTKAK SQISFYFNNE EEDNNKKKSS PATKVPSKPD RNSKILRTKL 480
RGEARGGAED AQKEQIRKEN QKKLHEKLEK NGLLRFSAAD ANGPDSEPRQ YFKKYESYVR 540
DSQLPTNIRD LRIHVDWKSQ TIILPIYGRP VPFHINSYKN GSKNEEGEYT YLRLNFNSPG 600
SSGGISKKVE ELPYEESADN QFVRSITLRS KDGDRMSETF KQIADLKKEA TKREQERKAL 660
ADVVQQDKLI ENKTGRTKRL DQIFVRPNPD TKRVPSTVFI HENGIRFQSP LRTDSRIDIL 720
FSNIKNLIFQ SCKGELIVVI HIHLKNPILM GKKKIQDVQF YREASDMSVD ETGGGRRGQS 780
RFRRYGDEDE LEQEQEERRK RAALDKEFKY FADAIAEASN GLLTVENTFR DLGFQGVPNR 840
SAVFCMPTTD CLVQLIEPPF LVINLEEVEI CILERVQFGL KNFDMVFVYK DFNKPVTHIN 900
TVPIESLDFL KQWLTDMDIP YTVSTINLNW ATIMKSLQDD PYQFFLDGGW NFLATGSDDE 960
ASDESEEEVS EYEASEDDVS DESAFSEDEE GSEVDDDISG DESEDYTGDE SEEGEDWDEL 1020
EKKAARADRG ANFRD 1035 
Gene Ontology
 GO:0035101; C:FACT complex; IGI:SGD.
 GO:0000790; C:nuclear chromatin; IDA:SGD.
 GO:0031298; C:replication fork protection complex; IDA:SGD.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0034724; P:DNA replication-independent nucleosome organization; IDA:SGD.
 GO:0006261; P:DNA-dependent DNA replication; IPI:SGD.
 GO:0006334; P:nucleosome assembly; IDA:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IDA:SGD.
 GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IMP:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IPI:SGD. 
Interpro
 IPR013719; DUF1747.
 IPR013953; FACT_Spt16p.
 IPR000994; Pept_M24_structural-domain. 
Pfam
 PF00557; Peptidase_M24
 PF08512; Rtt106
 PF08644; SPT16 
SMART
  
PROSITE
  
PRINTS