UniProt Accession

 ECM21_YEAST; P38167; D6VPQ2 

Genbank Protein ID

 X79489; Z35862; BK006936 

Genbank Nucleotide ID

 CAA55995.1; CAB39760.1; DAA07022.1 

Protein Name

 Protein ECM21 

Protein Synonyms/Alias

 Extracellular mutant protein 21 

Gene Name

 ECM21 

Gene Synonyms/Alias

 YBL101C; YBL0814 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
11	ITSRPVAKNSSHSLS	ubiquitination	[1]
26	ETDLNQSKGQPFQPS	ubiquitination	[2]
74	AVLNNTTKGGNNNGN	ubiquitination	[2]
94	TDAQLLGKKQNKQPP	ubiquitination	[2]
191	TRLANSKKFNEQFLI	ubiquitination	[1, 2, 3]
562	IFAEYPIKVIRTPPP	ubiquitination	[2]
577	VAVSTANKPIYINRV	ubiquitination	[2, 3, 4, 5, 6, 7]
617	IKLAPICKNVCVKRI	ubiquitination	[1, 2]
637	ERVTFVSKGYEYEYD	ubiquitination	[2]
651	DQTDPVAKDPYNPYY	ubiquitination	[2, 3, 4, 6, 7]
712	FDDDSDSKGNPKERL	ubiquitination	[3, 4, 6, 7]
784	IGFLSGHKGSKSHEE	ubiquitination	[1]
787	LSGHKGSKSHEENEK	ubiquitination	[1]
794	KSHEENEKPVYDPKF	ubiquitination	[1, 2, 4, 6, 7]
807	KFHQTIIKSNSGLPV	ubiquitination	[1, 2, 3, 4, 6, 7]
842	SNVYCRHKLEIMLRI	ubiquitination	[2]
851	EIMLRISKPDPECPS	ubiquitination	[2]
1024	SRFNNLDKLLSTPSP	ubiquitination	[2, 4, 6, 7]
1062	NATTENSKDKQNEFF	ubiquitination	[1, 2]
1064	TTENSKDKQNEFFKK	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149]
 [4] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [5] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [6] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [7] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 May be involved in cell wall organization and biogenesis. 

Sequence Annotation

 MOD_RES 56 56 Phosphoserine.
 MOD_RES 87 87 Phosphothreonine.
 MOD_RES 123 123 Phosphothreonine.
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 550 550 Phosphoserine.
 MOD_RES 775 775 Phosphoserine.
 MOD_RES 962 962 Phosphoserine.
 MOD_RES 1030 1030 Phosphoserine.
 MOD_RES 1035 1035 Phosphoserine.
 MOD_RES 1039 1039 Phosphoserine.
 CROSSLNK 577 577 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 651 651 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 712 712 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 794 794 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 807 807 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1024 1024 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Isopeptide bond; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1117 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0070086; P:ubiquitin-dependent endocytosis; IMP:SGD. 

Interpro

 IPR011022; Arrestin_C-like. 

Pfam

 PF02752; Arrestin_C 

SMART

 SM01017; Arrestin_C 

PROSITE

  

PRINTS