CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022639
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 EH domain-containing protein 3 
Protein Synonyms/Alias
  
Gene Name
 Ehd3 
Gene Synonyms/Alias
 Ehd2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
13GNDDRRKKDPEVFQTubiquitination[1]
26QTVSDGLKKLYKTKLubiquitination[1]
32LKKLYKTKLLPLEEYubiquitination[1]
58EDADFDNKPMVLLVGubiquitination[1]
124DPKKPFRKLNAFGNAubiquitination[1]
205DEFSEVIKALKNHEDubiquitination[1]
220KMRVVLNKADQIETQubiquitination[1]
269LLIPDNRKLFEAEEQubiquitination[1]
357PGDFPNLKRMQDQLQubiquitination[1]
370LQAQDFSKFQPLKSKubiquitination[1]
410QRPVQMVKGGAFEGTubiquitination[1]
475KKEMVRSKLPNSVLGubiquitination[1]
483LPNSVLGKIWKLADIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Plays a role in endocytic transport (By similarity). 
Sequence Annotation
 DOMAIN 444 532 EH.
 DOMAIN 476 511 EF-hand.
 NP_BIND 65 72 ATP (By similarity).
 BINDING 220 220 ATP (By similarity).
 BINDING 258 258 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 456 456 Phosphoserine.  
Keyword
 Acetylation; ATP-binding; Calcium; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Endosome; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 535 AA 
Protein Sequence
MFSWLGNDDR RKKDPEVFQT VSDGLKKLYK TKLLPLEEYY RFHEFHSPAL EDADFDNKPM 60
VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV MQGDVEGIIP GNALVVDPKK 120
PFRKLNAFGN AFLNRFVCAQ LPNAVLESIS VIDTPGILSG EKQRISRGYD FAAVLEWFAE 180
RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL 240
GKIVNTPEVI RVYIGSFWSH PLLIPDNRKL FEAEEQDLFR DIQSLPRNAA LRKLNDLIKR 300
ARLAKVHAYI ISSLKKEMPS VFGKDTKKKE LVNNLAEIYG RIEREHQISP GDFPNLKRMQ 360
DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE ETQRPVQMVK GGAFEGTLQG 420
PFGHGYGEGA GEGIDDAEWV VARDKPMYDE IFYTLSPVDG KITGANAKKE MVRSKLPNSV 480
LGKIWKLADI DKDGMLDDEE FALANHLIKV KLEGHELPSE LPAHLLPPSK RKVSE 535 
Gene Ontology
 GO:0030139; C:endocytic vesicle; IDA:MGI.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0032456; P:endocytic recycling; ISS:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0051260; P:protein homooligomerization; ISS:UniProtKB.
 GO:0072661; P:protein targeting to plasma membrane; IMP:MGI.
 GO:0086036; P:regulation of cardiac muscle cell membrane potential; IMP:MGI. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N 
SMART
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS