CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010387
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Dihydrofolate reductase 
Protein Synonyms/Alias
  
Gene Name
 folA 
Gene Synonyms/Alias
 dhfR; MPN_321; MP515 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
25NSLPWRIKAELQHFRacetylation[1]
133YTGDKYLKVDFSPFEacetylation[1]
143FSPFELTKEISFAEFacetylation[1]
157FKVAYYHKIAR****acetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819
Functional Description
 Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity). 
Sequence Annotation
 DOMAIN 1 160 DHFR.
 NP_BIND 6 7 NADP (By similarity).
 NP_BIND 14 19 NADP (By similarity).
 NP_BIND 43 46 NADP (By similarity).
 NP_BIND 62 65 NADP (By similarity).
 NP_BIND 101 106 NADP (By similarity).
 REGION 5 7 Substrate binding (By similarity).
 BINDING 27 27 Substrate (By similarity).
 BINDING 32 32 Substrate (By similarity).
 BINDING 57 57 Substrate (By similarity).
 BINDING 120 120 Substrate (By similarity).  
Keyword
 Complete proteome; NADP; One-carbon metabolism; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 160 AA 
Protein Sequence
MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL PPVFSERNVY 60
ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI CGGAQVYEQL IPRCDALIVS 120
TIFGKYTGDK YLKVDFSPFE LTKEISFAEF KVAYYHKIAR 160 
Gene Ontology
 GO:0004146; F:dihydrofolate reductase activity; IEA:EC.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR012259; DHFR.
 IPR024072; DHFR-like_dom.
 IPR017925; DHFR_CS.
 IPR001796; DHFR_dom. 
Pfam
 PF00186; DHFR_1 
SMART
  
PROSITE
 PS00075; DHFR_1
 PS51330; DHFR_2 
PRINTS
 PR00070; DHFR.