UniProt Accession

 AMPN_RAT; P15684; Q9JHP4 

Genbank Protein ID

 M25073; M26710; Y18765 

Genbank Nucleotide ID

 AAA41502.1; AAA57129.1; CAB93958.1 

Protein Name

 Aminopeptidase N 

Protein Synonyms/Alias

 AP-N; rAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Kidney Zn peptidase; KZP; Microsomal aminopeptidase; CD13 

Gene Name

 Anpep 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
826	TLVNEADKLRSALAC	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis (By similarity). 

Sequence Annotation

 REGION 33 68 Cytosolic Ser/Thr-rich junction.
 REGION 69 965 Metalloprotease.
 REGION 351 355 Substrate binding (By similarity).
 ACT_SITE 388 388 Proton acceptor (By similarity).
 METAL 387 387 Zinc; catalytic (By similarity).
 METAL 391 391 Zinc; catalytic (By similarity).
 METAL 410 410 Zinc; catalytic (By similarity).
 MOD_RES 176 176 Sulfotyrosine (Potential).
 MOD_RES 852 852 Phosphotyrosine (By similarity).
 CARBOHYD 114 114 N-linked (GlcNAc...) (Potential).
 CARBOHYD 128 128 N-linked (GlcNAc...) (Potential).
 CARBOHYD 234 234 N-linked (GlcNAc...) (Potential).
 CARBOHYD 242 242 N-linked (GlcNAc...) (Potential).
 CARBOHYD 264 264 N-linked (GlcNAc...) (Potential).
 CARBOHYD 555 555 N-linked (GlcNAc...) (Potential).
 CARBOHYD 606 606 N-linked (GlcNAc...) (Potential).
 CARBOHYD 624 624 N-linked (GlcNAc...) (Potential).
 CARBOHYD 780 780 N-linked (GlcNAc...) (Potential).
 DISULFID 760 767 By similarity.
 DISULFID 797 833 By similarity.  

Keyword

 Aminopeptidase; Angiogenesis; Complete proteome; Developmental protein; Differentiation; Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Reference proteome; Signal-anchor; Sulfation; Transmembrane; Transmembrane helix; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 965 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031983; C:vesicle lumen; IDA:RGD.
 GO:0012506; C:vesicle membrane; IDA:RGD.
 GO:0004177; F:aminopeptidase activity; IDA:RGD.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0042277; F:peptide binding; IDA:RGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0006725; P:cellular aromatic compound metabolic process; IDA:RGD.
 GO:0035814; P:negative regulation of renal sodium excretion; IMP:RGD.
 GO:0006508; P:proteolysis; IDA:RGD. 

Interpro

 IPR024571; DUF3358.
 IPR001930; Peptidase_M1.
 IPR014782; Peptidase_M1_N. 

Pfam

 PF11838; DUF3358
 PF01433; Peptidase_M1 

SMART

  

PROSITE

 PS00142; ZINC_PROTEASE 

PRINTS

 PR00756; ALADIPTASE.