UniProt Accession

 NNRD_HUMAN; Q8IW45; B4DXT4; Q5T9X3; Q9H7W1; Q9NVF5 

Genbank Protein ID

 AK001631; AK024260; AK302117; AL139385; CH471085; CH471085; BC041028 

Genbank Nucleotide ID

 BAA91797.1; BAB14863.1; BAG63496.1; CAI17008.1; EAX09121.1; EAX09125.1; AAH41028.1 

Protein Name

 ATP-dependent (S)-NAD(P)H-hydrate dehydratase 

Protein Synonyms/Alias

 ATP-dependent NAD(P)HX dehydratase; Carbohydrate kinase domain-containing protein 

Gene Name

 CARKD 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
172	QGILEVSKARDIPVV	ubiquitination	[1]
200	ALIHGYRKAVLTPNH	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity). 

Sequence Annotation

 DOMAIN 53 344 YjeF C-terminal.
 NP_BIND 246 250 ATP (By similarity).
 NP_BIND 265 274 ATP (By similarity).
 REGION 206 212 NAD(P)HX (By similarity).
 BINDING 153 153 NAD(P)HX; via amide nitrogen (By
 BINDING 275 275 NAD(P)HX (By similarity).
 MOD_RES 85 85 Phosphotyrosine (By similarity).
 CARBOHYD 240 240 N-linked (GlcNAc...).
 CARBOHYD 297 297 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Glycoprotein; Lyase; Mitochondrion; NAD; NADP; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 347 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047453; F:ATP-dependent NAD(P)H-hydrate dehydratase activity; IEA:HAMAP.
 GO:0046496; P:nicotinamide nucleotide metabolic process; IEA:HAMAP. 

Interpro

 IPR026599; NnrD/CARKD.
 IPR000631; YjeF_C_carb_kinase-rel. 

Pfam

 PF01256; Carb_kinase 

SMART

  

PROSITE

 PS01049; YJEF_C_1
 PS01050; YJEF_C_2
 PS51383; YJEF_C_3 

PRINTS