CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000652
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methylcytosine dioxygenase TET3 
Protein Synonyms/Alias
  
Gene Name
 TET3 
Gene Synonyms/Alias
 KIAA0401 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44GPWRVDQKPDWEAAPubiquitination[1]
156EWLEGKIKSVVMEGGubiquitination[1]
676KYLDTPTKSLLDTPAubiquitination[1]
884KEEEVLRKSFQDLATubiquitination[1, 2]
898TEVAPLYKRLAPQAYubiquitination[1]
1080GLSQQGLKPSLKVEPubiquitination[1, 2]
1456LPLGSSEKLFGALKSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O- GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. 
Sequence Annotation
 METAL 942 942 Iron; catalytic (By similarity).
 METAL 944 944 Iron; catalytic (By similarity).
 METAL 1538 1538 Iron; catalytic (By similarity).
 BINDING 1553 1553 2-oxoglutarate (By similarity).  
Keyword
 Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; Developmental protein; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1660 AA 
Protein Sequence
MDSGPVYHGD SRQLSASGVP VNGAREPAGP SLLGTGGPWR VDQKPDWEAA PGPAHTARLE 60
DAHDLVAFSA VAEAVSSYGA LSTRLYETFN REMSREAGNN SRGPRPGPEG CSAGSEDLDT 120
LQTALALARH GMKPPNCNCD GPECPDYLEW LEGKIKSVVM EGGEERPRLP GPLPPGEAGL 180
PAPSTRPLLS SEVPQISPQE GLPLSQSALS IAKEKNISLQ TAIAIEALTQ LSSALPQPSH 240
STPQASCPLP EALSPPAPFR SPQSYLRAPS WPVVPPEEHS SFAPDSSAFP PATPRTEFPE 300
AWGTDTPPAT PRSSWPMPRP SPDPMAELEQ LLGSASDYIQ SVFKRPEALP TKPKVKVEAP 360
SSSPAPAPSP VLQREAPTPS SEPDTHQKAQ TALQQHLHHK RSLFLEQVHD TSFPAPSEPS 420
APGWWPPPSS PVPRLPDRPP KEKKKKLPTP AGGPVGTEKA APGIKPSVRK PIQIKKSRPR 480
EAQPLFPPVR QIVLEGLRSP ASQEVQAHPP APLPASQGSA VPLPPEPSLA LFAPSPSRDS 540
LLPPTQEMRS PSPMTALQPG STGPLPPADD KLEELIRQFE AEFGDSFGLP GPPSVPIQDP 600
ENQQTCLPAP ESPFATRSPK QIKIESSGAV TVLSTTCFHS EEGGQEATPT KAENPLTPTL 660
SGFLESPLKY LDTPTKSLLD TPAKRAQAEF PTCDCVEQIV EKDEGPYYTH LGSGPTVASI 720
RELMEERYGE KGKAIRIEKV IYTGKEGKSS RGCPIAKWVI RRHTLEEKLL CLVRHRAGHH 780
CQNAVIVILI LAWEGIPRSL GDTLYQELTD TLRKYGNPTS RRCGLNDDRT CACQGKDPNT 840
CGASFSFGCS WSMYFNGCKY ARSKTPRKFR LAGDNPKEEE VLRKSFQDLA TEVAPLYKRL 900
APQAYQNQVT NEEIAIDCRL GLKEGRPFAG VTACMDFCAH AHKDQHNLYN GCTVVCTLTK 960
EDNRCVGKIP EDEQLHVLPL YKMANTDEFG SEENQNAKVG SGAIQVLTAF PREVRRLPEP 1020
AKSCRQRQLE ARKAAAEKKK IQKEKLSTPE KIKQEALELA GITSDPGLSL KGGLSQQGLK 1080
PSLKVEPQNH FSSFKYSGNA VVESYSVLGN CRPSDPYSMN SVYSYHSYYA QPSLTSVNGF 1140
HSKYALPSFS YYGFPSSNPV FPSQFLGPGA WGHSGSSGSF EKKPDLHALH NSLSPAYGGA 1200
EFAELPSQAV PTDAHHPTPH HQQPAYPGPK EYLLPKAPLL HSVSRDPSPF AQSSNCYNRS 1260
IKQEPVDPLT QAEPVPRDAG KMGKTPLSEV SQNGGPSHLW GQYSGGPSMS PKRTNGVGGS 1320
WGVFSSGESP AIVPDKLSSF GASCLAPSHF TDGQWGLFPG EGQQAASHSG GRLRGKPWSP 1380
CKFGNSTSAL AGPSLTEKPW ALGAGDFNSA LKGSPGFQDK LWNPMKGEEG RIPAAGASQL 1440
DRAWQSFGLP LGSSEKLFGA LKSEEKLWDP FSLEEGPAEE PPSKGAVKEE KGGGGAEEEE 1500
EELWSDSEHN FLDENIGGVA VAPAHGSILI ECARRELHAT TPLKKPNRCH PTRISLVFYQ 1560
HKNLNQPNHG LALWEAKMKQ LAERARARQE EAARLGLGQQ EAKLYGKKRK WGGTVVAEPQ 1620
QKEKKGVVPT RQALAVPTDS AVTVSSYAYT KVTGPYSRWI 1660 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0001939; C:female pronucleus; IEA:Compara.
 GO:0001940; C:male pronucleus; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0070579; F:methylcytosine dioxygenase activity; ISS:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0080111; P:DNA demethylation; ISS:UniProtKB.
 GO:0044727; P:DNA demethylation of male pronucleus; ISS:UniProtKB.
 GO:0080182; P:histone H3-K4 trimethylation; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0006493; P:protein O-linked glycosylation; IMP:UniProtKB. 
Interpro
 IPR024779; 2OGFeDO_nucleic_acid_mod. 
Pfam
 PF12851; Tet_JBP 
SMART
  
PROSITE
  
PRINTS