CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004221
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hematopoietic lineage cell-specific protein 
Protein Synonyms/Alias
 Hematopoietic cell-specific LYN substrate 1; LckBP1; p75 
Gene Name
 HCLS1 
Gene Synonyms/Alias
 HS1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
41KEQRWGAKTIEGSGRacetylation[1]
60NIHQLRNKVSEEHDVubiquitination[2]
123AAKGFGGKYGVERDRacetylation[1]
123AAKGFGGKYGVERDRubiquitination[2]
192ESQRDYAKGFGGQYGacetylation[1]
192ESQRDYAKGFGGQYGubiquitination[2]
241GTRGLKAKFESMAEEacetylation[1]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. 
Sequence Annotation
 REPEAT 79 115 Cortactin 1.
 REPEAT 116 152 Cortactin 2.
 REPEAT 153 189 Cortactin 3.
 REPEAT 190 212 Cortactin 4; truncated.
 DOMAIN 428 486 SH3.
 REGION 27 66 Involved in HAX-1 binding.
 MOD_RES 41 41 N6-acetyllysine.
 MOD_RES 103 103 Phosphotyrosine.
 MOD_RES 123 123 N6-acetyllysine.
 MOD_RES 140 140 Phosphotyrosine (By similarity).
 MOD_RES 192 192 N6-acetyllysine.
 MOD_RES 198 198 Phosphotyrosine.
 MOD_RES 222 222 Phosphotyrosine; by FGR.
 MOD_RES 241 241 N6-acetyllysine.
 MOD_RES 275 275 Phosphoserine.
 MOD_RES 308 308 Phosphothreonine.
 MOD_RES 378 378 Phosphotyrosine; by SYK and FES (By
 MOD_RES 397 397 Phosphotyrosine; by SYK and FES (By  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MWKSVVGHDV SVSVETQGDD WDTDPDFVND ISEKEQRWGA KTIEGSGRTE HINIHQLRNK 60
VSEEHDVLRK KEMESGPKAS HGYGGRFGVE RDRMDKSAVG HEYVAEVEKH SSQTDAAKGF 120
GGKYGVERDR ADKSAVGFDY KGEVEKHTSQ KDYSRGFGGR YGVEKDKWDK AALGYDYKGE 180
TEKHESQRDY AKGFGGQYGI QKDRVDKSAV GFNEMEAPTT AYKKTTPIEA ASSGTRGLKA 240
KFESMAEEKR KREEEEKAQQ VARRQQERKA VTKRSPEAPQ PVIAMEEPAV PAPLPKKISS 300
EAWPPVGTPP SSESEPVRTS REHPVPLLPI RQTLPEDNEE PPALPPRTLE GLQVEEEPVY 360
EAEPEPEPEP EPEPENDYED VEEMDRHEQE DEPEGDYEEV LEPEDSSFSS ALAGSSGCPA 420
GAGAGAVALG ISAVAVYDYQ GEGSDELSFD PDDVITDIEM VDEGWWRGRC HGHFGLFPAN 480
YVKLLE 486 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005667; C:transcription factor complex; IDA:BHF-UCL.
 GO:0071345; P:cellular response to cytokine stimulus; IMP:BHF-UCL.
 GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
 GO:2000107; P:negative regulation of leukocyte apoptotic process; IMP:BHF-UCL.
 GO:2000251; P:positive regulation of actin cytoskeleton reorganization; IC:BHF-UCL.
 GO:0008284; P:positive regulation of cell proliferation; ISS:UniProtKB.
 GO:0030854; P:positive regulation of granulocyte differentiation; IMP:BHF-UCL.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IMP:BHF-UCL.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IMP:BHF-UCL.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IMP:BHF-UCL.
 GO:0042993; P:positive regulation of transcription factor import into nucleus; IMP:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISS:UniProtKB.
 GO:0030833; P:regulation of actin filament polymerization; IMP:BHF-UCL.
 GO:0009725; P:response to hormone stimulus; ISS:UniProtKB. 
Interpro
 IPR003134; Hs1_Cortactin.
 IPR000108; p67phox.
 IPR001452; SH3_domain. 
Pfam
 PF02218; HS1_rep
 PF00018; SH3_1 
SMART
 SM00326; SH3 
PROSITE
 PS51090; CORTACTIN
 PS50002; SH3 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.