CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037067
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Methyl-CpG-binding domain protein 1 
Protein Synonyms/Alias
  
Gene Name
 PCM1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
409VLQEKKQKMDKLLGEubiquitination[1, 2]
412EKKQKMDKLLGELHTubiquitination[1, 2, 3, 4, 5]
489GHLNPSEKLQKLNEVubiquitination[4]
492NPSEKLQKLNEVRKRubiquitination[1, 2, 3, 5, 6]
658EDAEFEQKINRLMAAubiquitination[1, 2, 4, 5]
707YPAEEDTKQNSNNTRubiquitination[3, 4, 7]
722GNANKTQKDTGVNEKubiquitination[3]
729KDTGVNEKAREKFYEubiquitination[3]
733VNEKAREKFYEAKLQubiquitination[1, 2, 5]
738REKFYEAKLQQQQREubiquitination[1, 2, 3, 4, 5, 6, 7]
747QQQQRELKQLQEERKubiquitination[2, 5]
754KQLQEERKKLIDIQEubiquitination[2, 5]
755QLQEERKKLIDIQEKubiquitination[2, 5]
762KLIDIQEKIQALQTAubiquitination[2, 5, 6, 7]
786SVGNCPTKKYMPAVTubiquitination[2, 5, 6, 7]
787VGNCPTKKYMPAVTSubiquitination[1, 2, 3, 4, 5, 6]
807QHETSTSKSVFEPEDubiquitination[2, 3, 4, 5, 7]
841EELRQRRKQLEALMAubiquitination[1, 2, 3, 4, 5, 6]
954KETKNRWKNNCPFSAubiquitination[1, 2, 5]
970ENYRPLAKTRQQNISubiquitination[1, 2, 5, 6, 7]
996ELSYVEEKEQWQEQIubiquitination[2, 3, 5]
1007QEQINQLKKQLDFSVubiquitination[1, 2, 3]
1076QNNVQRLKQMLNELMubiquitination[1, 2, 3, 4, 5, 7, 8]
1092QQNQHPEKPGGKERGubiquitination[3]
1096HPEKPGGKERGSSASubiquitination[3]
1181TEYMAFPKPFESSSSubiquitination[1, 2, 3, 4, 5, 8]
1193SSSIGAEKPRNKKLPubiquitination[2, 3, 5, 8]
1197GAEKPRNKKLPEEEVubiquitination[2, 3, 4, 6]
1198AEKPRNKKLPEEEVEubiquitination[2, 3, 5, 6, 7]
1221EQEGEVEKPFIKTGFubiquitination[1, 2, 3, 4, 5, 7]
1225EVEKPFIKTGFSVSVubiquitination[4, 7]
1234GFSVSVEKSTSSNRKubiquitination[2, 5]
1275VDPTTVTKTFKTRKAubiquitination[2, 3]
1291AQASLASKDKTPKSKubiquitination[2, 3]
1296ASKDKTPKSKSKKRNubiquitination[2]
1329SSTCEPCKSRNRHSAubiquitination[2, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2016 AA 
Protein Sequence
MATGGGPFED GMNDQDLPNW SNENVDDRLN NMDWGAQQKK ANRSSEKNKK KFGVESDKRV 60
TNDISPESSP GVGRRRTKTP HTFPHSRYMS QMSVPEQAEL EKLKQRINFS DLDQRSIGSD 120
SQGRATAANN KRQLSENRKP FNFLPMQINT NKSKDASTNP PNRETIGSAQ CKELFASALS 180
NDLLQNCQVS EEDGRGEPAM ESSQIVSRLV QIRDYITKAS SMREDLVEKN ERSANVERLT 240
HLIDHLKEQE KSYMKFLKKI LARDPQQEPM EEIENLKKQH DLLKRMLQQQ EQLRALQGRQ 300
AALLALQHKA EQAIAVMDDS VVAETAGSLS GVSITSELNE ELNDLIQRFH NQLRDSQPPA 360
VPDNRRQAES LSLTREVSQS RKPSASERLP DEKVELFSKM RVLQEKKQKM DKLLGELHTL 420
RDQHLNNSSS SPQRSVDQRS TSAPSASVGL APVVNGESNS LTSSVPYPTA SLVSQNESEN 480
EGHLNPSEKL QKLNEVRKRL NELRELVHYY EQTSDMMTDA VNENRKDEET EESEYDSEHE 540
NSEPVTNIRN PQVASTWNEV NSHSNAQCVS NNRDGRTVNS NCEINNRSAA NIRALNMPPS 600
LDCRYNREGE QEIHVAQGED DEEEEEEAEE EGVSGASLSS HRSSLVDEHP EDAEFEQKIN 660
RLMAAKQKLR QLQDLVAMVQ DDDAAQGVIS ASASNLDDFY PAEEDTKQNS NNTRGNANKT 720
QKDTGVNEKA REKFYEAKLQ QQQRELKQLQ EERKKLIDIQ EKIQALQTAC PDLQLSAASV 780
GNCPTKKYMP AVTSTPTVNQ HETSTSKSVF EPEDSSIVDN ELWSEMRRHE MLREELRQRR 840
KQLEALMAEH QRRQGLAETA SPVAVSLRSD GSENLCTPQQ SRTEKTMATW GGSTQCALDE 900
EGDEDGYLSE GIVRTDEEEE EEQDASSNDN FSVCPSNSVN HNSYNGKETK NRWKNNCPFS 960
ADENYRPLAK TRQQNISMQR QENLRWVSEL SYVEEKEQWQ EQINQLKKQL DFSVSICQTL 1020
MQDQQTLSCL LQTLLTGPYS VMPSNVASPQ VHFIMHQLNQ CYTQLTWQQN NVQRLKQMLN 1080
ELMRQQNQHP EKPGGKERGS SASHPPSPSL FCPFSFPTQP VNLFNIPGFT NFSSFAPGMN 1140
FSPLFPSNFG DFSQNISTPS EQQQPLAQNS SGKTEYMAFP KPFESSSSIG AEKPRNKKLP 1200
EEEVESSRTP WLYEQEGEVE KPFIKTGFSV SVEKSTSSNR KNQLDTNGRR RQFDEESLES 1260
FSSMPDPVDP TTVTKTFKTR KASAQASLAS KDKTPKSKSK KRNSTQLKSR VKNIRYESAS 1320
MSSTCEPCKS RNRHSAQTEE PVQAKVFSRK NHEQLEKIIK CNRSTEISSE TGSDFSMFEA 1380
LRDTIYSEVA TLISQNESRP HFLIELFHEL QLLNTDYLRQ RALYALQDIV SRHISESHEK 1440
GENVKSVNSG TWIASNSELT PSESLATTDD ETFEKNFERE THKISEQNDA DNASVLSVSS 1500
NFEPFATDDL GNTVIHLDQA LARMREYERM KTEAESNSNM RCTCRIIEDG DGAGAETPVI 1560
ENRSSQQPVS EVSTIPCPRI DTQQLDRQIK AIMKEVIPFL KEHMDEVCSS QLLTSVRRMV 1620
LTLTQQNDES KEFVKFFHKQ LGSILQDSLA KFAGRKLKDC GEDLLVEISE VLFNELAFFK 1680
LMQDLDNNSI TVKQRCKRKI EATGVIQSCA KEAKRILEDH GSPAGEIDDE DKDKDETETV 1740
KQTQTSEVYD GPKNVRSDIS DQEEDEESEG CPVSINLSKA ETQALTNYGS GEDENEDEEM 1800
EEFEEGPVDV QTSLQANTEA TEENEHDEQV LQRDFKKTAE SKNVPLEREA TSKNDQNNCP 1860
VKPCYLNILE DEQPLNSAAH KESPPTVDST QQPNPLPLRL PEMEPLVPRV KEVKSAQETP 1920
ESSLAGSPDT ESPVLVNDYE AESGNISQKS DEEDFVKVED LPLKLTIYSE ADLRKKMVEE 1980
EQKNHLSGEI CEMQTEELAG NSETLKEPET VGAQSI 2016 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA. 
Interpro
 IPR024138; Pericentriolar_Pcm1. 
Pfam
  
SMART
  
PROSITE
  
PRINTS