UniProt Accession

 DPYS_MOUSE; Q9EQF5; Q99PP1; Q9DBK3; Q9DBP7 

Genbank Protein ID

 AF249296; AF227731; AK004822; AK004899; BC037086 

Genbank Nucleotide ID

 AAG37999.1; AAK00644.1; BAB23593.1; BAB23654.1; AAH37086.1 

Protein Name

 Dihydropyrimidinase 

Protein Synonyms/Alias

 DHP; DHPase; Dihydropyrimidine amidohydrolase; Hydantoinase 

Gene Name

 Dpys 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
57	RILDAAGKLVLPGGI	ubiquitination	[1]
159	DKGVNSFKMFMAYKG	ubiquitination	[1]
256	VMSKSAAKVVADARR	acetylation	[2]
256	VMSKSAAKVVADARR	phosphoglycerylation	[3]
256	VMSKSAAKVVADARR	succinylation	[2]
256	VMSKSAAKVVADARR	ubiquitination	[1]
339	TCQKALGKDDFTKIP	ubiquitination	[1]
344	LGKDDFTKIPNGVNG	acetylation	[4]
344	LGKDDFTKIPNGVNG	ubiquitination	[1]
362	RMSVIWEKGVHSGKM	ubiquitination	[1]
384	VTSTNAAKIFNLYPK	ubiquitination	[1]
391	KIFNLYPKKGRIAVG	ubiquitination	[1]
489	TCTPVPVKRAPYKGE	ubiquitination	[1]
494	PVKRAPYKGEVTTLK	ubiquitination	[1]
501	KGEVTTLKARETKED	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6- dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate (By similarity). 

Sequence Annotation

 METAL 67 67 Zinc 1 (By similarity).
 METAL 69 69 Zinc 1 (By similarity).
 METAL 159 159 Zinc 1; via carbamate group (By
 METAL 159 159 Zinc 2; via carbamate group (By
 METAL 192 192 Zinc 2 (By similarity).
 METAL 248 248 Zinc 2 (By similarity).
 METAL 326 326 Zinc 1 (By similarity).
 BINDING 164 164 Substrate (By similarity).
 BINDING 347 347 Substrate; via carbonyl oxygen (By
 MOD_RES 159 159 N6-carboxylysine (By similarity).  

Keyword

 Complete proteome; Hydrolase; Metal-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 519 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:BHF-UCL.
 GO:0016597; F:amino acid binding; ISS:BHF-UCL.
 GO:0004157; F:dihydropyrimidinase activity; ISS:BHF-UCL.
 GO:0002059; F:thymine binding; ISS:BHF-UCL.
 GO:0002058; F:uracil binding; ISS:BHF-UCL.
 GO:0008270; F:zinc ion binding; ISS:BHF-UCL.
 GO:0019482; P:beta-alanine metabolic process; ISS:BHF-UCL.
 GO:0051289; P:protein homotetramerization; ISS:BHF-UCL.
 GO:0006210; P:thymine catabolic process; ISS:BHF-UCL.
 GO:0006212; P:uracil catabolic process; ISS:BHF-UCL. 

Interpro

 IPR006680; Amidohydro_1.
 IPR011778; Hydantoinase/dihydroPyrase.
 IPR011059; Metal-dep_hydrolase_composite. 

Pfam

 PF01979; Amidohydro_1 

SMART

  

PROSITE

  

PRINTS