CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023240
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting nexin-6 
Protein Synonyms/Alias
 TRAF4-associated factor 2; Sorting nexin-6, N-terminally processed 
Gene Name
 SNX6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53VKFTVHTKSSLPNFKubiquitination[1]
60KSSLPNFKQNEFSVVubiquitination[1, 2, 3, 4, 5]
109ASREKLQKLGEGEGSubiquitination[1, 3]
119EGEGSMTKEEFTKMKubiquitination[1, 3, 4, 6]
126KEEFTKMKQELEAEYubiquitination[1]
138AEYLAIFKKTVAMHEubiquitination[2, 5]
183RGKNKKEKLEDFFKNubiquitination[1, 3]
189EKLEDFFKNMVKSADubiquitination[1, 2, 5]
193DFFKNMVKSADGVIVubiquitination[1, 3]
204GVIVSGVKDVDDFFEubiquitination[1, 3, 4]
239DRMTRSHKSAADDYNubiquitination[3]
265QDSTDICKFFLKVSEubiquitination[1]
269DICKFFLKVSELFDKubiquitination[1, 3, 4]
276KVSELFDKTRKIEARubiquitination[1, 3]
291VSADEDLKLSDLLKYubiquitination[1, 3]
297LKLSDLLKYYLRESQubiquitination[1]
307LRESQAAKDLLYRRSubiquitination[1, 3]
325VDYENANKALDKARAubiquitination[1]
333ALDKARAKNKDVLQAubiquitination[1]
335DKARAKNKDVLQAETubiquitination[1]
350SQQLCCQKFEKISESubiquitination[1]
353LCCQKFEKISESAKQubiquitination[1]
359EKISESAKQELIDFKubiquitination[1, 3]
366KQELIDFKTRRVAAFubiquitination[1, 3]
375RRVAAFRKNLVELAEubiquitination[1, 2, 3, 4, 5]
386ELAELELKHAKGNLQubiquitination[1, 4]
389ELELKHAKGNLQLLQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May be involved in several stages of intracellular trafficking. Promotes lysosomal degradation of CDKN1B (By similarity). Plays a role in retrograde protein transport from endosomes to the trans-Golgi network. May function as link between transport vesicles and dynactin. Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network. May contribute to transcription regulation. 
Sequence Annotation
 DOMAIN 26 173 PX.
 REGION 41 47 Phosphatidylinositol bisphosphate binding
 REGION 100 106 Phosphatidylinositol bisphosphate binding
 REGION 114 117 Phosphatidylinositol bisphosphate binding
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 2 2 N-acetylmethionine; in Sorting nexin-6,  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endosome; Lipid-binding; Membrane; Nucleus; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 406 AA 
Protein Sequence
MMEGLDDGPD FLSEEDRGLK AINVDLQSDA ALQVDISDAL SERDKVKFTV HTKSSLPNFK 60
QNEFSVVRQH EEFIWLHDSF VENEDYAGYI IPPAPPRPDF DASREKLQKL GEGEGSMTKE 120
EFTKMKQELE AEYLAIFKKT VAMHEVFLCR VAAHPILRRD LNFHVFLEYN QDLSVRGKNK 180
KEKLEDFFKN MVKSADGVIV SGVKDVDDFF EHERTFLLEY HNRVKDASAK SDRMTRSHKS 240
AADDYNRIGS SLYALGTQDS TDICKFFLKV SELFDKTRKI EARVSADEDL KLSDLLKYYL 300
RESQAAKDLL YRRSRSLVDY ENANKALDKA RAKNKDVLQA ETSQQLCCQK FEKISESAKQ 360
ELIDFKTRRV AAFRKNLVEL AELELKHAKG NLQLLQNCLA VLNGDT 406 
Gene Ontology
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0031901; C:early endosome membrane; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IMP:UniProtKB.
 GO:0007175; P:negative regulation of epidermal growth factor-activated receptor activity; NAS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:HGNC.
 GO:0042147; P:retrograde transport, endosome to Golgi; IMP:UniProtKB. 
Interpro
 IPR027267; AH/BAR-dom.
 IPR001683; Phox.
 IPR014637; Snx5_Snx6.
 IPR015404; Vps5_C. 
Pfam
 PF00787; PX
 PF09325; Vps5 
SMART
  
PROSITE
 PS50195; PX 
PRINTS