CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022910
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translocation protein SEC63 homolog 
Protein Synonyms/Alias
  
Gene Name
 SEC63 
Gene Synonyms/Alias
 SEC63L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
75PNIIPTVKKIVLLAGubiquitination[1]
76NIIPTVKKIVLLAGWubiquitination[1]
121GATVAEIKKQYRLLSubiquitination[2]
130QYRLLSLKYHPDKGGubiquitination[3, 4]
135SLKYHPDKGGDEVMFubiquitination[3, 4, 5]
147VMFMRIAKAYAALTDubiquitination[1, 2, 3, 4, 5]
288IGSINLKKNEPPLTCubiquitination[5]
300LTCPYSLKARVLLLSubiquitination[3, 4]
313LSHLARMKIPETLEEubiquitination[5]
327EDQQFMLKKCPALLQubiquitination[1, 2]
368ASLENCMKLSQMAVQubiquitination[6]
381VQGLQQFKSPLLQLPubiquitination[3, 4]
407NHKKYKIKTIQDLVSubiquitination[3, 4]
416IQDLVSLKESDRHTLubiquitination[2]
633ALLETKSKITHPVYSubiquitination[3, 4]
688LKFPAPGKPGNYQYTubiquitination[3, 4]
710YMGLDQIKPLKLEVHubiquitination[3, 4]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Required for integral membrane and secreted preprotein translocation across the endoplasmic reticulum membrane. 
Sequence Annotation
 DOMAIN 104 165 J.
 DOMAIN 197 541 SEC63 1.
 DOMAIN 637 714 SEC63 2.
 MOD_RES 307 307 Phosphoserine (By similarity).
 MOD_RES 593 593 Phosphoserine (By similarity).
 MOD_RES 597 597 Phosphoserine (By similarity).
 MOD_RES 742 742 Phosphoserine.
 MOD_RES 748 748 Phosphoserine.  
Keyword
 Chaperone; Coiled coil; Complete proteome; Disease mutation; Endoplasmic reticulum; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 760 AA 
Protein Sequence
MAGQQFQYDD SGNTFFYFLT SFVGLIVIPA TYYLWPRDQN AEQIRLKNIR KVYGRCMWYR 60
LRLLKPQPNI IPTVKKIVLL AGWALFLFLA YKVSKTDREY QEYNPYEVLN LDPGATVAEI 120
KKQYRLLSLK YHPDKGGDEV MFMRIAKAYA ALTDEESRKN WEEFGNPDGP QATSFGIALP 180
AWIVDQKNSI LVLLVYGLAF MVILPVVVGS WWYRSIRYSG DQILIRTTQI YTYFVYKTRN 240
MDMKRLIMVL AGASEFDPQY NKDATSRPTD NILIPQLIRE IGSINLKKNE PPLTCPYSLK 300
ARVLLLSHLA RMKIPETLEE DQQFMLKKCP ALLQEMVNVI CQLIVMARNR EEREFRAPTL 360
ASLENCMKLS QMAVQGLQQF KSPLLQLPHI EEDNLRRVSN HKKYKIKTIQ DLVSLKESDR 420
HTLLHFLEDE KYEEVMAVLG SFPYVTMDIK SQVLDDEDSN NITVGSLVTV LVKLTRQTMA 480
EVFEKEQSIC AAEEQPAEDG QGETNKNRTK GGWQQKSKGP KKTAKSKKKK PLKKKPTPVL 540
LPQSKQQKQK QANGVVGNEA AVKEDEEEVS DKGSDSEEEE TNRDSQSEKD DGSDRDSDRE 600
QDEKQNKDDE AEWQELQQSI QRKERALLET KSKITHPVYS LYFPEEKQEW WWLYIADRKE 660
QTLISMPYHV CTLKDTEEVE LKFPAPGKPG NYQYTVFLRS DSYMGLDQIK PLKLEVHEAK 720
PVPENHPQWD TAIEGDEDQE DSEGFEDSFE EEEEEEEDDD 760 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0004872; F:receptor activity; TAS:ProtInc.
 GO:0031204; P:posttranslational protein targeting to membrane, translocation; IEA:InterPro.
 GO:0006612; P:protein targeting to membrane; TAS:ProtInc. 
Interpro
 IPR001623; DnaJ_domain.
 IPR027137; Sec63.
 IPR004179; Sec63-dom. 
Pfam
 PF00226; DnaJ
 PF02889; Sec63 
SMART
 SM00271; DnaJ
 SM00611; SEC63
 SM00973; Sec63 
PROSITE
 PS00636; DNAJ_1
 PS50076; DNAJ_2 
PRINTS
 PR00625; JDOMAIN.