CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012015
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translation initiation factor eIF-2B subunit epsilon 
Protein Synonyms/Alias
 eIF-2B GDP-GTP exchange factor subunit epsilon 
Gene Name
 EIF2B5 
Gene Synonyms/Alias
 EIF2BE 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61RRFFPISKDQPRVLLubiquitination[1]
103CWKAAQIKEHLLKSKubiquitination[1]
141VLRDVDAKALVRSDFubiquitination[1, 2]
186SVMTMIFKESSPSHPubiquitination[1]
217NRVLHFQKTQGLRRFubiquitination[1]
420LCDNAEVKERVTLKPubiquitination[1]
481EKDKVKMKGYNPAEVubiquitination[1]
493AEVGAAGKGYLWKAAubiquitination[1, 3, 4]
693SQRDTTDKGQQLRKNubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. 
Sequence Annotation
 DOMAIN 543 720 W2.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 544 544 Phosphoserine; by DYRK2.
 MOD_RES 717 717 Phosphoserine.
 MOD_RES 718 718 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Disease mutation; Initiation factor; Leukodystrophy; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 721 AA 
Protein Sequence
MAAPVVAPPG VVVSRANKRS GAGPGGSGGG GARGAEEEPP PPLQAVLVAD SFDRRFFPIS 60
KDQPRVLLPL ANVALIDYTL EFLTATGVQE TFVFCCWKAA QIKEHLLKSK WCRPTSLNVV 120
RIITSELYRS LGDVLRDVDA KALVRSDFLL VYGDVISNIN ITRALEEHRL RRKLEKNVSV 180
MTMIFKESSP SHPTRCHEDN VVVAVDSTTN RVLHFQKTQG LRRFAFPLSL FQGSSDGVEV 240
RYDLLDCHIS ICSPQVAQLF TDNFDYQTRD DFVRGLLVNE EILGNQIHMH VTAKEYGARV 300
SNLHMYSAVC ADVIRRWVYP LTPEANFTDS TTQSCTHSRH NIYRGPEVSL GHGSILEENV 360
LLGSGTVIGS NCFITNSVIG PGCHIGDNVV LDQTYLWQGV RVAAGAQIHQ SLLCDNAEVK 420
ERVTLKPRSV LTSQVVVGPN ITLPEGSVIS LHPPDAEEDE DDGEFSDDSG ADQEKDKVKM 480
KGYNPAEVGA AGKGYLWKAA GMNMEEEEEL QQNLWGLKIN MEEESESESE QSMDSEEPDS 540
RGGSPQMDDI KVFQNEVLGT LQRGKEENIS CDNLVLEINS LKYAYNISLK EVMQVLSHVV 600
LEFPLQQMDS PLDSSRYCAL LLPLLKAWSP VFRNYIKRAA DHLEALAAIE DFFLEHEALG 660
ISMAKVLMAF YQLEILAEET ILSWFSQRDT TDKGQQLRKN QQLQRFIQWL KEAEEESSED 720
D 721 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IMP:UniProtKB.
 GO:0016740; F:transferase activity; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; NAS:UniProtKB.
 GO:0031369; F:translation initiation factor binding; ISS:UniProtKB.
 GO:0014002; P:astrocyte development; IMP:UniProtKB.
 GO:0042552; P:myelination; IMP:UniProtKB.
 GO:0032057; P:negative regulation of translational initiation in response to stress; ISS:UniProtKB.
 GO:0014003; P:oligodendrocyte development; IMP:UniProtKB.
 GO:0001541; P:ovarian follicle development; IMP:UniProtKB.
 GO:0045948; P:positive regulation of translational initiation; ISS:UniProtKB.
 GO:0009749; P:response to glucose stimulus; ISS:UniProtKB.
 GO:0009408; P:response to heat; IMP:UniProtKB.
 GO:0043434; P:response to peptide hormone stimulus; ISS:UniProtKB.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR001451; Hexapep_transf.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR011004; Trimer_LpxA-like.
 IPR003307; W2_domain. 
Pfam
 PF00132; Hexapep
 PF02020; W2 
SMART
 SM00515; eIF5C 
PROSITE
 PS51363; W2 
PRINTS