CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014513
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase Praja-2 
Protein Synonyms/Alias
 Praja2 
Gene Name
 Pja2 
Gene Synonyms/Alias
 Neurodap1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
46RHAYVSFKPCMTRHEacetylation[1]
336GFNRHEAKQRSVQRWacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA- mediated long-term memory processes. 
Sequence Annotation
 ZN_FING 633 674 RING-type; atypical.
 REGION 530 707 Interaction with PRKAR1A, PRKAR2A and
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 306 306 Phosphoserine (By similarity).
 MOD_RES 320 320 Phosphoserine (By similarity).
 MOD_RES 339 339 Phosphoserine; by PKA (By similarity).
 MOD_RES 430 430 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Ligase; Membrane; Metal-binding; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Synapse; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 707 AA 
Protein Sequence
MSQYTEKEPS VMDQDSSKAA WPRAAGGYQT ITGRRYGRRH AYVSFKPCMT RHERSLGRAG 60
DDYEVLELDD VAKENTAGSS SLDQVHPSLP SETTVEKSET EIPTCGPALN QSTESNPSVA 120
TVCHSEEVRE TLDSSTNLQN HAERECTPAV CNASSVQNGI VLVHTDSYDP DSKHDENDSL 180
QLCAQAVEGG RRQKVLGNAV FELENGEVER YADLCPSVPS LSGEIREESE ELGSALLEKN 240
SAGDAEAVHQ DGQEFQRSSE DGIVRKRRQD DTDQGRQTEN STEDADCVPG HVEQNTSERA 300
NHHGSSPEQV VRPKVRKVIS SSQVDQESGF NRHEAKQRSV QRWREALEVE ECSSDDPIIK 360
CDDYDGDHDC MFLTPSYSRV TPREAERHRA TAENGATASG RQEARENAFW NACGEYYQLF 420
DKDEDSSECS DGEWSASLPH RFSGTEKDQS SSDESWETLP GKDENEPELQ SDSSGPEEEN 480
QELSLQEGEQ TSLEEGEIPW LQYNEVNESS SDEGNEPANE FAQPEAFMLD GNNNLEDDSS 540
VSEDLDVDWS LFDGFADGLG VAEAISYVDP QFLTYMALEE RLAQAMETAL AHLESLAVDV 600
EVANPPASKE SIDGLPETLV LEDHTAIGQE QCCPICCSEY IKDDIATELP CHHFFHKPCV 660
SIWLQKSGTC PVCRRHFPPA VIDASAAASS EPDLDASPAN DNAEEAP 707 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0034236; F:protein kinase A catalytic subunit binding; ISS:UniProtKB.
 GO:0034237; F:protein kinase A regulatory subunit binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007616; P:long-term memory; IMP:UniProtKB.
 GO:0010738; P:regulation of protein kinase A signaling cascade; IMP:UniProtKB. 
Interpro
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF13639; zf-RING_2 
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS