UniProt Accession

 EI3JA_MOUSE; Q3UGC7 

Genbank Protein ID

 AK147154; AK148004; AK154934; AK161038; AL845457; CH466519 

Genbank Nucleotide ID

 BAE27721.1; BAE28282.1; BAE32935.1; BAE36162.1; CAM21780.1; EDL28081.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit J-A 

Protein Synonyms/Alias

 eIF3j-A; Eukaryotic translation initiation factor 3 subunit 1-A; eIF-3-alpha-A; eIF3 p35 

Gene Name

 Eif3j1 

Gene Synonyms/Alias

 Eif3s1-1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
120	PEEQLADKLRLKKLQ	acetylation	[1, 2]
120	PEEQLADKLRLKKLQ	ubiquitination	[3]
164	DDFTEFGKLLKDKIT	ubiquitination	[3]
201	LEIDDLKKITNSLTV	acetylation	[4]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA (By similarity). 

Sequence Annotation

 REGION 4 72 Sufficient for interaction with EIF3B (By
 REGION 246 261 Promotes stable association with the 40S
 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 16 16 Phosphoserine (By similarity).
 MOD_RES 23 23 Phosphoserine (By similarity).
 MOD_RES 112 112 Phosphothreonine (By similarity).
 MOD_RES 130 130 Phosphoserine (By similarity).
 MOD_RES 257 257 Phosphotyrosine (By similarity).  

Keyword

 Coiled coil; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IEA:HAMAP.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 

Interpro

 IPR023194; eIF3-like_dom.
 IPR013906; eIF3j. 

Pfam

 PF08597; eIF3_subunit 

SMART

  

PROSITE

  

PRINTS