UniProt Accession

 THIM_HUMAN; P42765; Q9BUT6 

Genbank Protein ID

 D16294; BC001918 

Genbank Nucleotide ID

 BAA03800.1; AAH01918.1 

Protein Name

 3-ketoacyl-CoA thiolase, mitochondrial 

Protein Synonyms/Alias

 Acetyl-CoA acyltransferase; Beta-ketothiolase; Mitochondrial 3-oxoacyl-CoA thiolase; T1 

Gene Name

 ACAA2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	GAYGGLLKDFTATDL	acetylation	[1, 2]
38	DLSEFAAKAALSAGK	acetylation	[3]
81	GLRVGIPKETPALTI	acetylation	[1]
137	RNVRFGTKLGSDIKL	acetylation	[1]
181	ISREECDKYALQSQQ	acetylation	[1]
234	TTLEQLQKLPPVFKK	acetylation	[2]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Abolishes BNIP3-mediated apoptosis and mitochondrial damage. 

Sequence Annotation

 ACT_SITE 92 92 Acyl-thioester intermediate (By
 ACT_SITE 352 352 Proton acceptor (By similarity).
 ACT_SITE 382 382 Proton acceptor (By similarity).
 MOD_RES 119 119 Phosphothreonine.
 MOD_RES 121 121 Phosphoserine.
 MOD_RES 127 127 Phosphotyrosine.
 MOD_RES 137 137 N6-acetyllysine (By similarity).
 MOD_RES 270 270 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Lipid metabolism; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 397 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0003988; F:acetyl-CoA C-acyltransferase activity; NAS:UniProtKB.
 GO:0006695; P:cholesterol biosynthetic process; NAS:UniProtKB.
 GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-UniPathway.
 GO:1901029; P:negative regulation of mitochondrial outer membrane permeabilization; IDA:UniProtKB. 

Interpro

 IPR002155; Thiolase.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr.
 IPR020615; Thiolase_acyl_enz_int_AS.
 IPR020610; Thiolase_AS.
 IPR020617; Thiolase_C.
 IPR020613; Thiolase_CS.
 IPR020616; Thiolase_N. 

Pfam

 PF02803; Thiolase_C
 PF00108; Thiolase_N 

SMART

  

PROSITE

 PS00098; THIOLASE_1
 PS00737; THIOLASE_2
 PS00099; THIOLASE_3 

PRINTS