UniProt Accession

 SAP30_HUMAN; O75446; D3DP38 

Genbank Protein ID

 AF055993; CH471056; CH471056; BC016757 

Genbank Nucleotide ID

 AAC33316.1; EAX04755.1; EAX04756.1; AAH16757.1 

Protein Name

 Histone deacetylase complex subunit SAP30 

Protein Synonyms/Alias

 30 kDa Sin3-associated polypeptide; Sin3 corepressor complex subunit SAP30; Sin3-associated polypeptide p30 

Gene Name

 SAP30 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
104	KVKIELDKSARHLYI	acetylation	[1]
104	KVKIELDKSARHLYI	ubiquitination	[2, 3]
194	RSIPVNEKDTLTYFI	ubiquitination	[3]
214	DKNKSDLKVDSGVH*	ubiquitination	[2, 3, 4]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Involved in the functional recruitment of the Sin3- histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N- CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. 

Sequence Annotation

 ZN_FING 67 115 Atypical.
 REGION 1 129 Interaction with NCOR1 (By similarity).
 REGION 130 220 Interaction with SIN3A (By similarity).
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 138 138 Phosphoserine.
 MOD_RES 145 145 Phosphothreonine.  

Keyword

 3D-structure; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 220 AA 

Protein Sequence

Gene Ontology

 GO:0000118; C:histone deacetylase complex; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR024145; His_deAcase_SAP30/SAP30L.
 IPR025718; SAP30_Sin3-bd.
 IPR025717; SAP30_zn-finger. 

Pfam

 PF13867; SAP30_Sin3_bdg
 PF13866; zf-SAP30 

SMART

  

PROSITE

  

PRINTS