CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004141
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sodium transport ATPase 1 
Protein Synonyms/Alias
  
Gene Name
 ENA1 
Gene Synonyms/Alias
 HOR6; PMR2; PMR2A; YDR040C; YD6888.02C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
123TKTMNSLKNLSSPNAubiquitination[1]
137AHVIRNGKSETINSKubiquitination[1]
144KSETINSKDVVPGDIubiquitination[1]
170DLRLIETKNFDTDESubiquitination[1]
196DANLVFGKEEETSVGubiquitination[1, 2]
235ALNSEIGKIAKSLQGubiquitination[1]
238SEIGKIAKSLQGDSGubiquitination[1]
253LISRDPSKSWLQNTWubiquitination[1]
353SRNVIVRKLDSLEALubiquitination[1]
370VNDICSDKTGTLTQGubiquitination[1]
378TGTLTQGKMLARQIWubiquitination[1]
440FKDRLYEKDLPEDIDubiquitination[1]
523SLSQHNEKPGSAQFEubiquitination[1]
581WYGKDGVKITPLTDCubiquitination[1]
617FASKSFTKDQVNDDQubiquitination[1]
626QVNDDQLKNITSNRAubiquitination[1]
750EALHRRKKFCTMTGDubiquitination[1]
1068NDLESNNKRDPFEAYubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538] 
Functional Description
 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the sodium or lithium ions to allow salt tolerance. Is negatively modulated by SIS2/HAL3. 
Sequence Annotation
 ACT_SITE 369 369 4-aspartylphosphate intermediate (By  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Hydrolase; Ion transport; Magnesium; Membrane; Nucleotide-binding; Reference proteome; Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1091 AA 
Protein Sequence
MGEGTTKENN NAEFNAYHTL TAEEAAEFIG TSLTEGLTQD EFVHRLKTVG ENTLGDDTKI 60
DYKAMVLHQV CNAMIMVLLI SMIISFAMHD WITGGVISFV IAVNVLIGLV QEYKATKTMN 120
SLKNLSSPNA HVIRNGKSET INSKDVVPGD ICLVKVGDTI PADLRLIETK NFDTDESLLT 180
GESLPVSKDA NLVFGKEEET SVGDRLNLAF SSSAVVKGRA KGIVIKTALN SEIGKIAKSL 240
QGDSGLISRD PSKSWLQNTW ISTKKVTGAF LGTNVGTPLH RKLSKLAVLL FWIAVLFAII 300
VMASQKFDVD KRVAIYAICV ALSMIPSSLV VVLTITMSVG AAVMVSRNVI VRKLDSLEAL 360
GAVNDICSDK TGTLTQGKML ARQIWIPRFG TITISNSDDP FNPNEGNVSL IPRFSPYEYS 420
HNEDGDVGIL QNFKDRLYEK DLPEDIDMDL FQKWLETATL ANIATVFKDD ATDCWKAHGD 480
PTEIAIQVFA TKMDLPHNAL TGEKSTNQSN ENDQSSLSQH NEKPGSAQFE HIAEFPFDST 540
VKRMSSVYYN NHNETYNIYG KGAFESIISC CSSWYGKDGV KITPLTDCDV ETIRKNVYSL 600
SNEGLRVLGF ASKSFTKDQV NDDQLKNITS NRATAESDLV FLGLIGIYDP PRNETAGAVK 660
KFHQAGINVH MLTGDFVGTA KAIAQEVGIL PTNLYHYSQE IVDSMVMTGS QFDGLSEEEV 720
DDLPVLPLVI ARCSPQTKVR MIEALHRRKK FCTMTGDGVN DSPSLKMANV GIAMGINGSD 780
VSKEASDIVL SDDNFASILN AVEEGRRMTD NIQKFVLQLL AENVAQALYL IIGLVFRDEN 840
GKSVFPLSPV EVLWIIVVTS CFPAMGLGLE KAAPDLMDRP PHDSEVGIFT WEVIIDTFAY 900
GIIMTGSCMA SFTGSLYGIN SGRLGHDCDG TYNSSCRDVY RSRSAAFATM TWCALILAWE 960
VVDMRRSFFR MHPDTDSPVK EFFRSIWGNQ FLFWSIIFGF VSAFPVVYIP VINDKVFLHK 1020
PIGAEWGLAI AFTIAFWIGA ELYKCGKRRY FKTQRAHNPE NDLESNNKRD PFEAYSTSTT 1080
IHTEVNIGIK Q 1091 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0008556; F:potassium-transporting ATPase activity; IMP:SGD.
 GO:0008554; F:sodium-exporting ATPase activity, phosphorylative mechanism; IDA:SGD.
 GO:0042149; P:cellular response to glucose starvation; IMP:SGD.
 GO:0006972; P:hyperosmotic response; IGI:SGD.
 GO:0009268; P:response to pH; IGI:SGD.
 GO:0009651; P:response to salt stress; IDA:UniProtKB. 
Interpro
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR006414; ATPase_P-typ_Na/Ca.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.