UniProt Accession

 ICAL_MOUSE; P51125; Q9EQV4; Q9EQV5; Q9QXQ3; Q9QXQ4; Q9R0N1 

Genbank Protein ID

 AB026997; AF190152; AF190151; AB044334; AB044334; AB044334; AB044334; X62519 

Genbank Nucleotide ID

 BAA84768.1; AAF25194.1; AAF25193.1; BAB18888.1; BAB18889.1; BAB18886.1; BAB18887.1; CAA44385.1 

Protein Name

 Calpastatin 

Protein Synonyms/Alias

 Calpain inhibitor 

Gene Name

 Cast 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
30	TQEHVNEKNIGSSSK	acetylation	[1]
96	TKAIPVNKQLEGPDQ	acetylation	[1]
129	EQPVVHEKKSKGGPK	acetylation	[2]
204	TVATALDKVVGKKKE	acetylation	[1, 3]
341	KSTGEIFKAQSAGVT	ubiquitination	[4]
464	DRSTYQDKPSTPAEK	acetylation	[2]
504	PPKLASLKGVVPEDA	ubiquitination	[4]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. 

Sequence Annotation

 REPEAT 251 303 Inhibitory domain 1.
 REPEAT 384 436 Inhibitory domain 2.
 REPEAT 524 577 Inhibitory domain 3.
 REPEAT 661 714 Inhibitory domain 4.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 219 219 Phosphoserine.
 MOD_RES 324 324 Phosphoserine (By similarity).
 MOD_RES 444 444 Phosphoserine (By similarity).
 MOD_RES 446 446 Phosphoserine (By similarity).
 MOD_RES 453 453 Phosphoserine (By similarity).
 MOD_RES 479 479 Phosphothreonine.
 MOD_RES 653 653 Phosphoserine (By similarity).
 MOD_RES 655 655 Phosphoserine (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Phosphoprotein; Protease inhibitor; Reference proteome; Repeat; Thiol protease inhibitor. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 788 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IDA:MGI.
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
 GO:0010466; P:negative regulation of peptidase activity; IGI:MGI.
 GO:0030163; P:protein catabolic process; ISA:MGI. 

Interpro

 IPR026998; Calpastatin.
 IPR001259; Prot_inh_calpain. 

Pfam

 PF00748; Calpain_inhib 

SMART

  

PROSITE

  

PRINTS