UniProt Accession

 EIF3D_HUMAN; O15371; B2R7D4; Q3MJD9; Q5M9Q6 

Genbank Protein ID

 U54558; BT007381; CR456489; AK312939; AL022313; CH471095; BC000328; BC000469; BC014912; BC080515; BC093100; BC093686; BC101477 

Genbank Nucleotide ID

 AAD03466.1; AAP36045.1; CAG30375.1; BAG35781.1; CAA18440.1; EAW60109.1; AAH00328.1; AAH00469.1; AAH14912.1; AAH80515.1; AAH93100.1; AAH93686.1; AAI01478.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit D 

Protein Synonyms/Alias

 eIF3d; Eukaryotic translation initiation factor 3 subunit 7; eIF-3-zeta; eIF3 p66 

Gene Name

 EIF3D 

Gene Synonyms/Alias

 EIF3S7 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
35	MPYQPFSKGDRLGKV	ubiquitination	[1, 2, 3, 4]
41	SKGDRLGKVADWTGA	ubiquitination	[3, 5, 6]
53	TGATYQDKRYTNKYS	ubiquitination	[1, 3, 4, 5, 6]
124	FNLQILPKSAKQKER	ubiquitination	[6]
127	QILPKSAKQKERERI	ubiquitination	[3]
139	ERIRLQKKFQKQFGV	ubiquitination	[3]
142	RLQKKFQKQFGVRQK	ubiquitination	[1, 3, 4]
171	VRSDWEVKEEMDFPQ	ubiquitination	[3]
204	GALEYYDKAFDRITT	ubiquitination	[3]
275	VVQRVGSKLFFDKRD	ubiquitination	[3]
280	GSKLFFDKRDNSDFD	ubiquitination	[3, 5]
350	FVEDDMDKNEIASVA	ubiquitination	[5, 6]
364	AYRYRRWKLGDDIDL	ubiquitination	[5]
426	AVIATELKNNSYKLA	ubiquitination	[3, 5]
431	ELKNNSYKLARWTCC	ubiquitination	[3]
505	CMKLEEGKYLILKDP	ubiquitination	[5]
510	EGKYLILKDPNKQVI	ubiquitination	[3, 5]
514	LILKDPNKQVIRVYS	ubiquitination	[3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 MOD_RES 161 161 Phosphoserine.
 MOD_RES 528 528 Phosphoserine.
 MOD_RES 529 529 Phosphoserine.
 MOD_RES 548 548 Phosphothreonine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 548 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001732; P:formation of translation initiation complex; IEA:Compara.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR007783; eif3d. 

Pfam

 PF05091; eIF-3_zeta 

SMART

  

PROSITE

  

PRINTS