CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006547
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bromodomain-containing factor 1 
Protein Synonyms/Alias
  
Gene Name
 BDF1 
Gene Synonyms/Alias
 YLR399C; L8084.18 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
299TIHPPKSKDIYPYESacetylation[1]
627GSRKRRSKALSQEEQubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Transcription factor involved in the expression of a broad class of genes including snRNAs. Required for sporulation and DNA-damage repair. Prevents the spreading of SIR silencing at telomeres and protects histone H4, but not H3, from deacetylation. 
Sequence Annotation
 DOMAIN 165 237 Bromo 1.
 DOMAIN 332 404 Bromo 2.
 DOMAIN 518 598 NET.
 MOD_RES 270 270 Phosphoserine.
 MOD_RES 429 429 Phosphoserine.
 MOD_RES 615 615 Phosphoserine.
 MOD_RES 659 659 Phosphoserine.  
Keyword
 Bromodomain; Coiled coil; Complete proteome; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Repeat; Sporulation; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 686 AA 
Protein Sequence
MTDITPVQND VDVNGNNVND DVSSNLKRPI DQGDPSNGLA EEENPANNQL HLKKARLDGD 60
ALTSSPAGLA ENGIEGATLA ANGENGYNAT GSGAEDEQQG LKKEEGGQGT KQEDLDENSK 120
QELPMEVPKE PAPAPPPEPD MNNLPQNPIP KHQQKHALLA IKAVKRLKDA RPFLQPVDPV 180
KLDIPFYFNY IKRPMDLSTI ERKLNVGAYE VPEQITEDFN LMVNNSIKFN GPNAGISQMA 240
RNIQASFEKH MLNMPAKDAP PVIAKGRRSS AQEDAPIVIR RAQTHNGRPK RTIHPPKSKD 300
IYPYESKKPK SKRLQQAMKF CQSVLKELMA KKHASYNYPF LEPVDPVSMN LPTYFDYVKE 360
PMDLGTIAKK LNDWQYQTME DFERDVRLVF KNCYTFNPDG TIVNMMGHRL EEVFNSKWAD 420
RPNLDDYDSD EDSRTQGDYD DYESEYSESD IDETIITNPA IQYLEEQLAR MKVELQQLKK 480
QELEKIRKER RLARGSKKRG KRSKGRSGSK NASSKGRRDK KNKLKTVVTY DMKRIITERI 540
NDLPTSKLER AIDIIKKSMP NISEDDEVEL DLDTLDNHTI LTLYNTFFRQ YESSSGASNG 600
LDGTSGVTRD ASSLSPTSAG SRKRRSKALS QEEQSRQIEK IKNKLAILDS ASPLSQNGSP 660
GQIQSAAHNG FSSSSDDDVS SESEEE 686 
Gene Ontology
 GO:0000812; C:Swr1 complex; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0001047; F:core promoter binding; IDA:SGD.
 GO:0070577; F:histone acetyl-lysine binding; IDA:SGD.
 GO:0001094; F:TFIID-class transcription factor binding; IDA:SGD.
 GO:0006338; P:chromatin remodeling; IPI:SGD.
 GO:0006281; P:DNA repair; IMP:SGD.
 GO:0031452; P:negative regulation of heterochromatin assembly; IMP:SGD.
 GO:1900051; P:positive regulation of histone exchange; IMP:SGD.
 GO:0090054; P:regulation of chromatin silencing at silent mating-type cassette; IMP:SGD.
 GO:0031938; P:regulation of chromatin silencing at telomere; IMP:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
 GO:0009301; P:snRNA transcription; IMP:SGD.
 GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. 
Interpro
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR027353; NET_dom. 
Pfam
 PF00439; Bromodomain 
SMART
 SM00297; BROMO 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51525; NET 
PRINTS
 PR00503; BROMODOMAIN.